军事医学2016，Vol.40Issue(7)：554-557,4.DOI:10.7644/j.issn.1674-9960.2016.07.005
应用DNA四面体探针电化学生物传感器检测埃博拉病毒核酸
An electrochemical biosensor based on a DNA tetrahedral nanostructure probe for the detection of Ebola virus nucleotide
摘要
Abstract
Objective To establish a quick electrochemical biosensor for the detection of nucleic acid of Ebola virus . Methods The DNA tetrahedral nanostructure was self-assembled on gold surface by strong Au-S chemical bonds , leaving the target probe at the top .A biotinylated-ssDNA was introduced as the detection probe by specific binding of the captured target sequence , before avidin-horseradish peroxidase ( HRP) was used as a signal amplifier to transduce amperometric sig-nal through interactions with TMB substrate .Results The results indicated that the nucleotide sequence of Ebola virus could be recognized and detected by the sensor .The linear range for the detection of target DNA was from 1.0 ×10 -9 to 5.0 ×10 -6 mol/L,and the detection limit was 5.2 ×10 -10 mol/L.Conclusion The fabricated sensor is demonstrated to be sensitive and specific for the detection of Ebola virus nucleotide .关键词
DNA四面体/电化学生物传感器/埃博拉病毒Key words
DNA tetrahedron/electrochemical biosensor/Ebola virus分类
医药卫生引用本文复制引用
王金艳,孔文,董世彪,贾雷立,刘超,郝荣章,宋宏彬..应用DNA四面体探针电化学生物传感器检测埃博拉病毒核酸[J].军事医学,2016,40(7):554-557,4.基金项目
国家863计划资助项目（2015AA020929）；北京市科技新星计划资助项目（ Z141107001814071）；国家传染病重大专项资助项目（AWS13C007，AWS15J006）；军事医学科学院创新基金资助项目 （）
