军事医学2016，Vol.40Issue(7)：561-563,568,4.DOI:10.7644/j.issn.1674-9960.2016.07.007
人乳腺癌肿瘤移植裸鼠模型的构建及其肿瘤浸润髓系细胞的分离
Construction of human breast cancer tumor transplantation model in nude mice and isolation of tumor infiltrating myeloid cells
摘要
Abstract
Objective To establish neoplasm transplantation models of breast cancer cells in BALB /c nude mice and to isolate tumor infiltrating myeloid cells.Methods pHAGE-EF-ZsG-DEST plasmid,pMD2.G plasmid and psPAX2 were transfected into BT474 using the method of calcium phosphate transfection .The positive cells were selected by flow cytometry and implanted in the fat pad of nude mice .A tumor model of breast cancer cells implanted in nude mice was constructed, and the tumor infiltrating myeloid cells were isolated .Conclusion Tumor infiltrating myeloid cells are successfully isolated, which will contribute to the study of the functions of tumor infiltrating myeloid cells .关键词
乳腺肿瘤/肿瘤移植/鼠/模型/肿瘤浸润/髓系细胞/慢病毒Key words
breast neoplasms/neoplasm transplantation/mice/models/neoplasm invasiveness/myeloid cells/lentivirus分类
医药卫生引用本文复制引用
王兴,柳迪,唐丽,贺福初..人乳腺癌肿瘤移植裸鼠模型的构建及其肿瘤浸润髓系细胞的分离[J].军事医学,2016,40(7):561-563,568,4.基金项目
国家自然科学基金资助项目（31570901，31270953）；国家973计划资助项目 （）
