江西农业大学学报2016,Vol.38Issue(3):488-491,4.
江西奉新县猕猴桃叶斑病病原菌鉴定
Identification of Pathogenic Fungus Causing Kiwifruit Leaf Spot in Fengxin County of Jiangxi Province
摘要
Abstract
In order to ascertain the fungal species responsible for the new kiwifruit leaf disease in Fengxin County of Jiangxi Province,a fungus was isolated from the diseased leaf samples by using the tissue isolation method.The obtained fungus was identified based on morphology and molecular biology,and its pathogenicity was tested.In total,32 fungal isolates with pathogenicity in artificially inoculation test were obtained.The cultur-al characters and conidial morphology and size of each isolate were identical, and consistent with those of Corynespora cassiicola in literature. Three isolates were randomly selected for analyzing their rDNA-ITS se-quences.The three rDNA-ITS sequences obtained were the same and identical to that of C. cassiicola. Thus,it may be concluded that the pathogen of kiwifruit leaf spot disease in Fengxin County of Jiangxi Province is Corynespora cassiicola,a species of genus Corynespora in fungi imperfect.关键词
猕猴桃叶斑病/病原鉴定/多主棒孢Key words
kiwifruit leaf spot/identification of pathogen/Corynespora cassiicola分类
农业科技引用本文复制引用
秦双林,王园秀,蒋军喜,崔朝宇,欧阳慧,黄婷..江西奉新县猕猴桃叶斑病病原菌鉴定[J].江西农业大学学报,2016,38(3):488-491,4.基金项目
国家自然科学基金项目(31460452)、江西省科技支撑项目(20141BBF60019)和江西省教育厅项目( GJJ13269) Project supported by the National Natural Science Foundation of China (31460452),the Science and Technology Department of Jiangxi Province ( 20141BBF60019 ) and the Education Department of Jiangxi Province ( GJJ13269) (31460452)