江西农业大学学报2016，Vol.38Issue(3)：542-548,7.
八角莲肉桂醇脱氢酶基因的克隆和表达分析
Cloning and Tissue Expression of A Cinnamyl Alcohol Dehydrogenase Gene from Dysosma versipellis
摘要
Abstract
Objective Podophyllotoxin is an important bioactive substance in D. versipellis.The gene encoding a cinnamyl alcohol dehydrogenase from root of D. versipellis was cloned, and its tissue expression pattern was characterized.Methods A CAD gene was obtained with degenerated primers amplification,3′RACE and 5′RACE from root cDNA,and analyzed by bioinformatic softwares.The expression pattern was predicted through cDNA am-plification from root,stem and leaves. Results A 1098 bp CAD gene encoding a 365 amino acid sequence was cloned.This amino acid sequence contained no plastid targeted signal peptide,had 67.25% similarities with the CAD protein from Populus tomentosa and Arabidopsis thaliana,and could be clustered with AtCAD7,AtCAD8 and AtCAD6 into the same group in phylogenetic analysis.The expression of the CAD gene was detected in root and stem,but not obviously in leaves of D.versipellis.Conclusion CAD is a key enzyme in biosynthesis of podo-phyllotoxin.The CAD gene from D. versipellis was cloned,which lays a foundation for podophyllotoxin genetic engineering.关键词
八角莲/鬼臼毒素/生物合成途径/CAD基因/克隆/表达Key words
Dysosma versipellis/podophyllotoxin/biosynthsis pathway/CAD gene/cloning/expression分类
农业科技引用本文复制引用
叶耀辉,郑红梅,王婷,马越兴,张博文,王晓云..八角莲肉桂醇脱氢酶基因的克隆和表达分析[J].江西农业大学学报,2016,38(3):542-548,7.基金项目
江西省教育厅科学技术研究项目( 2012A035) Project supported by the Science and Technology Support Projects of Jiangxi Provincial Education Department ( 2012A035) （ 2012A035）
