检验医学与临床2016，Vol.13Issue(14)：1911-1913,3.DOI:10.3969/j.issn.1672-9455.2016.14.003
流式免疫多重微球芯片技术检测干燥综合征自身抗体的评价及应用
Establishment and clinical application of multiplex flow cytometric immuno-bead array assay technology in detecting primary sjogren′s syndrome
摘要
Abstract
Objective To establish and applicata a method of multiplex flow cytometric immuno‐bead array assay technology in detecting primary sjogren′s syndrome (pSS) .Methods By multiplex flow cytometric immuno‐bead array assay technology ,polysty‐rene microspheres were coated by eight monoclonal antibodies [SSA (60 kD ) ,SSB ,α‐fodrin ,M3 ,ds‐DNA ,nRNP/Sm ,Sm ,M2 ] , which were also regarded as reaction interface .And the varieties of soluble protein were detected in one sample at the same time by this kind of method .A total of 206 pSS patients ,110 non‐pSS patients and 96 healthy people were selected as subjects of our study . The new method was used for detecting 8 autoantibodies in the pSS group ,non‐pSS group and normal group .Results The coeffi‐cient of variation (CV) of intra‐repetition and inter‐repetition were small in difference in detection of 8 autoantibodies ;compared with the pSS group ,the difference in the non‐pSS group and normal group had statistical significance(P<0 .01) .The sensitivity for the diagnosis of pSS group was 97 .20% and specificity was 96 .34% .Conclusion Multiplex flow cytometric immuno‐bead array as‐say technology can be used to detect eight antibodies of pSS ,which might provide for clinical diagnosis of pSS and other autoimmune diseases a quick and accurate method .关键词
原发性干燥综合征/多重微球芯片流式免疫荧光发光法/血清生物标志物/自身抗原/自身抗体Key words
primary sjogren′s syndrome/multiplex flow cytometric immuno-bead array assay technology/serum biomark-ers/autoantigen/autoantibodies引用本文复制引用
牛广华,高玉洁,张程,张艺凡,吕丹,崔百慧..流式免疫多重微球芯片技术检测干燥综合征自身抗体的评价及应用[J].检验医学与临床,2016,13(14):1911-1913,3.基金项目
辽宁省自然基金课题（2014010058-301）。 （）
