检验医学与临床2016，Vol.13Issue(14)：1927-1928,2.DOI:10.3969/j.issn.1672-9455.2016.14.009
烟曲霉的微管蛋白基因鉴定及唑类抗真菌药物的耐药性研究
Identification of Aspergillus fumigatus through tubulin gene amplification and drug resistance to antifungal azoles agents
摘要
Abstract
Objective To identify Aspergillus fumigatus through method of tubulin gene amplification and analyse drug resist‐ance to azole antifungal agents .Methods By using cetyltrimethyl ammonium bromide (CTAB) method ,genomic DNA from asper‐gillus fumigatus were extracted .Tubulin gene was amplified by polymerase chain reaction amplification assay and series analysis . Drug resistance of aspergillus fumigatus to antifungal azoles agents were determined by agar dilution method .Results A toal of 91 strains separated from specimen were identified as aspergillus fumigatus through phenotypic identification ,which grew at 35 ℃and 48 ℃ .Through tubulin gene amplification ,88 (96 .70% ) of the 91 strains were confirmed as aspergillus fumigatus .And 87 strains of the 88 Aspergillus fumigatus were confirmed sensitive to 4 μg/mL itraconazole and 1 μg/mL voriconazole ,and 1 strain was re‐sistant to 4 μg/mL itraconazole and 1 μg/mL voriconazole .Conclusion Aspergillus fumigatus could be identified by method of tu‐bulin gene amplification accurately .关键词
烟曲霉/伊曲康唑/伏立康唑/耐药性Key words
Aspergillus fumigatus/itraconazole/voriconazole/drug resistance引用本文复制引用
邹自英,刘媛,曾平,朱冰..烟曲霉的微管蛋白基因鉴定及唑类抗真菌药物的耐药性研究[J].检验医学与临床,2016,13(14):1927-1928,2.
