昆明医科大学学报2016，Vol.37Issue(6)：13-17,5.
龙血素A对大鼠肝星状细胞增殖及Frizzled-4受体蛋白表达的影响
Effect of Loureirin A on Proliferation and Frizzled-4 Expression of Rat Hepatic Stellate Cells in vitro
摘要
Abstract
Objective To investigate the molecular mechanism of Loureirin A mediated anti-hepatic fibrosis by evaluting its effects on proliferation , secretion ofα-smooth muscle actin (α-SMA) and transforming growth factor-beta1 (TGF-β1), and expression of rat hepatic stellate cells in vitro . Methods Primary hepatic stellate cells were isolated and cultured from Sprague-Dawley rats. After activating and inducing primary hepatic stellate cells from qHSC to aHSC, the activated hepatic stellate cells model in vitro was established. Then we observed the morphological changes of static hepatic stellate cells and activated hepatic stellate cells with inverted phase contrast microscope. Cultured hepatic stellate cells were treated with different concentrations of loureirin A and the inhibitory rate of HSCs proliferation was measured by MTT assay. The expression of Frizzled-4 was measured by western blot analysis. The content ofα-SMA and TGF-β1 in the cultured HSCs'supernatant were measured by enzyme-linked immunosorbent assay (ELISA) . Results Loureirin A the proliferation of inhibited activated hepatic stellate cells in a time-dose-dependent manner compared with the control group,IC50=0.30 μg/μL. After loureirinA treatment of the HSCs, western blot analysis showed that Frizzled-4 expression level was obviously lower than control group. Loureirin A also inhibitedα-SMA and TGFβ1 (P<0.05) secretion in the cultured HSCs'supernatant in different degree by the assay of ELISA. Conclusions The molecular mechanism of Loureirin A and Wnt signaling pathway mediated anti-hepatic fibrosis and anti-angiogenesis may involve down-regulation the expression of Frizzled-4, inhibiting the synthesis and secretion ofα-SMA,TGF-β1and the proliferation of HSCs.关键词
龙血素A/肝星状细胞/Frizzled-4受体蛋白/WNT信号通路/抗肝纤维化Key words
Loureirin A/Hepatic stellate cells/Frizzled-4/WNT signaling pathway/Anti-hepatic fibro-sis分类
医药卫生引用本文复制引用
胡建鹏,宋正己,寻琳婷,李霆,赵雪茹..龙血素A对大鼠肝星状细胞增殖及Frizzled-4受体蛋白表达的影响[J].昆明医科大学学报,2016,37(6):13-17,5.基金项目
国家自然科学基金资助项目（81560107）；云南省应用基础研究基金资助项目（昆明医科大学联合专项） （）
