临床口腔医学杂志2016，Vol.32Issue(7)：399-402,4.DOI:10.3969/j.issn.1003-1634.2016.07.005
N-乙酰基半胱氨酸对牙科水门汀生物相容性的影响
The effect of N-acetyl cysteine (NAC) on biocompatibility of dental cements
摘要
Abstract
Objective: To evaluate the potential amelioration of the cytotoxicity of dental cements as follows: Zinc Phosphate Cements(Zpc), Glass Ionomer Cements(FujiⅠ),Resin Modified Glass Ionomer(Fuji plus)and Resin Cements (G-cem) by N-acetyl cysteine (NAC) in vitro. Method:Four cements (Zpc,FujiⅠ,Fuji plus,G-cem) were cured according to the manufacturers’ instructions and extraction mediums were prepared. 3T3-Swiss albino(3T3) cells were exposed to the extrac-tion mediums in the presence of different concentration NAC (0mM,1mM,5mM,10mM,15mM). Cytotoxicity was measured by a modified 3-(4, 5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide(MTT) assay. Result: Four tested cements ex-hibited varying degrees of cytotoxic effect, and induced the reduction of cell relative growth rate which were 94.72 %, 80.89%, 31.44 %, 3.89 % respectively. The performance of cements were variable in the present of different concentration NAC. The RGR of FujiⅠand Fuji Plus appeared an increasing tendency with 1mM and 5 mM NAC, but there were no statistical difference. Instead, the RGR was lower when added 15 mM NAC (P<0.05). NAC which were added in the group of G-cem and ZPC exhibited notable detoxification effects and improved the RGR. Meanwhile 5 mM and 10 mM NAC had the best re-sults among different concentration (P < 0.05).Conclusion:The biocompatibility of different dental cements were great different, so pay attention to the toxicity of cements when select the cements in clinical practice. This study demonstrated that NAC can improve the biocompatibility of dental cements which were in a concentration dependent manner. And Further studies are needed to explore the mechanisms.关键词
N-乙酰基半胱氨酸/牙科水门汀/生物相容性Key words
N-acetyl cysteine/Dental cements/Biocompatibility分类
医药卫生引用本文复制引用
左陈启,王吓勇,朱亚桥,唐尤超,王远勤..N-乙酰基半胱氨酸对牙科水门汀生物相容性的影响[J].临床口腔医学杂志,2016,32(7):399-402,4.基金项目
广东惠州市科技计划项目 （）
