岭南现代临床外科2016，Vol.16Issue(4)：391-396,6.DOI:10.3969/j.issn.1009-976X.2016.04.005
miR-20b 在乳腺癌的表达及对乳腺癌细胞增殖的影响
Expressions and its role of miR-20b in breast cancer
唐炜 1叶熹罡 1潘凌霄 1高进 1陈伦 1张晓申 1郑文博1
作者信息
- 1. 510120 广州 广州医科大学附属第一医院乳腺外科
- 折叠
摘要
Abstract
Objective To investigate the expressions of miR-20b in breast cancer and its role in breast cancer cells in vitro. Methods Total RNA from 134 breast cancer tissues were isolated for miR-20b quantitative analysis by real-time RT-PCR. The associations between miR-20b expression levels and clinicopathological features were analyzed. Breast cancer cells were transfected with miR-20b inhibitors,then CCK-8 and flow cytometry methods were used to detect the proliferation and apoptosis of the cells. Results The expression of miR-20b was up-regulated in breast cancer tissues compared with those in adjacent tissues (P<0.001). There was a significant correlation between miR-20b expression and tumor stage,distal metastasis and Ki67 expression (P<0.05). High miR-20b expression was positively correlated with shorter DFS and OS (P<0.05). The down-expression of miR-20b resulted in decrease in the rate of cell proliferation and increase in cell apoptosis. Conclusion Higher miR-20b is associated with poor prognosis of breast cancer and may serve as independent prognostic markers for breast cancer cells.关键词
乳腺癌/实时定量RT-PCR/miR-20bKey words
Breast cancer/RT-PCR/miR-20b分类
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唐炜,叶熹罡,潘凌霄,高进,陈伦,张晓申,郑文博..miR-20b 在乳腺癌的表达及对乳腺癌细胞增殖的影响[J].岭南现代临床外科,2016,16(4):391-396,6.
