国际眼科杂志2016，Vol.16Issue(8)：1439-1443,5.DOI:10.3980/j.issn.1672-5123.2016.8.09
IL－10基因修饰的未成熟树突状细胞在大鼠角膜移植排斥反应中的作用
Role and its mechanism of immature dendritic cells with IL-10 gene modified in rats after keratoplasty
摘要
Abstract
Abstract? AIM: Through the establishment of penetrating keratoplasty model of rats, to detect the role and its mechanism of immature dendritic cells with IL-10 gene modified.? METHODS: Allogeneic penetrating corneal transplantation in rat model was performed. SD rats were randomly divided into positive control group, GFP-DC group, 8-DC and IL-10-GFP-DC group.At 3d before keratoplasty, the rats were given tail intravenous injection with the same amount of PBS, bone marrow 8-DC ( DC had cultured for 8d ) from donor Wistar rats, GFP-DC after 48h transfection and IL-10-GFP-DC.Rats were observed under slit-lamp for corneal graft cases every day, and recorded rejection index and corneal graft survival time.At 14d after keratoplasty, pathologic and immunohistochemical examinations were performed.?RESULTS:Compared with GFP-DC group and 8-DC group, corneal graft survival time of IL-10-GFP-DC group was significantly longer ( P<0.01 ); at 14d after keratoplasty, corneal opacity, edema, neovascularization and rejection index of IL-10 -GFP-DC group were significantly lower (P<0.01).Pathological examination showed that in the three experimental groups corneal inflammation was lighter than the positive control group without significant central graft neovascularization. Immunohistochemistry showed: compared to the positive control group, GFP-DC group and 8-DC group, CD4+, CD8+, CD25+, IL-2+, NK+and NF-κB+positive cells in IL-10-GFP-DC group were lower(P<0.01).? CONCLUSION: After donor -derived immature dendritic cells pretreated, corneal graft survival was significantly prolonged, successfully induced corneal transplantation tolerance. CD4+, CD8+, CD25+, IL-2+, NK+and NF-κB+positive cells are involved in corneal allograft rejection regulation, IL-10-GFP-DC may reduce CD4+, CD8+, CD25+, IL-2+, NK+and NF-κB+positive cell infiltration, inhibit corneal transplant rejection.关键词
IL-10/树突状细胞/穿透性角膜移植/移植排斥Key words
Interleukin-10/dendritic cells/penetrating keratoplasty/transplant rejection引用本文复制引用
李佳,李雪,孙建华,李兵..IL－10基因修饰的未成熟树突状细胞在大鼠角膜移植排斥反应中的作用[J].国际眼科杂志,2016,16(8):1439-1443,5.基金项目
辽宁省自然科学基金计划项目（ No．2013022001）@@@@ Project of Natural Science Foundation of Liaoning Province （）
