生态与农村环境学报2016，Vol.32Issue(4)：664-669,6.DOI:10.11934/j.issn.1673-4831.2016.04.023
施用畜禽粪便有机肥土壤抗生素抗性基因污染状况
Pollution of Antibiotics Resistant Genes in Farmland Amended With Livestock Organic Manure
摘要
Abstract
In order to investigate distribution of antibiotics resistant genes ( ARGs) in farmland soil amended with organic manure, soil samples from greenhouses and fields in nine vegetable production bases in the suburbs of Beijing were collect⁃ed for analysis of antibiotics resistant bacteria and 18 types of ARGs. The analysis shows that the soil samples from green⁃houses were higher than those from fields in proportion of tetracycline resistant bacteria to total bacteria in count ( P<0�05) , and little difference was found between the two groups of soil samples in proportion of ampicillin, sulfa methox⁃azole and ciprofloxacin resistant bacteria to total bacteria in count. Sulfanilamide resistant genes ( sulⅠand sul2) and tet⁃racycline resistance genes ( tetL) were detected in all the soil samples, whereas tetracycline resistant genes ( tetA, tetA/P and tetC) , erythromycin resistant genes ( ermB) , and I integron ( intⅠ1) were detected with a higher detectable rate in greenhouse soil than in field soil, which may be associated with more organic manure applied to the former than to the latter.关键词
土壤/有机肥/抗生素耐药菌/抗生素抗性基因Key words
soil/organic manure/antibiotics resistant bacteria/antibiotics resistant genes分类
资源环境引用本文复制引用
张兰河,王佳佳,高敏,仇天雷,韩梅琳,王旭明..施用畜禽粪便有机肥土壤抗生素抗性基因污染状况[J].生态与农村环境学报,2016,32(4):664-669,6.基金项目
家禽产业技术体系北京市创新团队专项资金（ CARS-PSTP）；北京市农林科学院科技创新能力建设专项 （）
