中南医学科学杂志2016，Vol.44Issue(3)：263-266,295,5.DOI:10.15972/j.cnki.43-1509/r.2016.03.006
梅毒螺旋体纤连蛋白结合蛋白Tp0136前炎症活性的研究
Proinflammatory Activity of Recombinant Fibronectin-Binding Proteins Tp0136 of Treponema Pallidum
摘要
Abstract
Objective To explore potential proinflammatory activity of the Treponema pallidum fibronectin-binding protein ( FnBP ) Tp0136. Methods The recombinant pET-28a/Tp0136 was constructed and induced to express the targeting protein in E.coli BL21.In vitro various concentrations of protein was used to stimulate human macrophages derived from THP-1 cell line at different time.ELISA was performed to detect the level of proinflammatory cytokines IL-1β,IL-6 and TNF-α;Recombinant protein was used to stimulate the macrophages pre-treated with PDTC,and ELISA was performed to de-tect the releasing IL-1β,IL-6 and TNF-α respectively. Results The recombinant plasmid was constructed and the corre-sponding recombinant protein was obtained successfully;rTp0136 could induce production of IL-1β,IL-6 and TNF-αin dose-and time-dependent manners significantly.Secretion of IL-1β,IL-6 and TNF-α decreased to 32%,35% and 27% respectively when macrophages was pre-treated with PDTC. Conclusion The data suggested that the FnBP Tp0136 could activate the THP-derived human macrophages to release the proinflammatory cytokines,which might be regulated via NF-κB.关键词
梅毒螺旋体/纤连蛋白结合蛋白/Tp0136/前炎症细胞因子/核因子kappa BKey words
Treponema pallidum/fibronectin-binding protein ( FnBP )/Tp0136/proinflammatory cytokines/NF-κB分类
医药卫生引用本文复制引用
肖勇健,刘卓然,赵飞骏,余坚,曾铁兵..梅毒螺旋体纤连蛋白结合蛋白Tp0136前炎症活性的研究[J].中南医学科学杂志,2016,44(3):263-266,295,5.基金项目
湖南省医药卫生科研计划项目( No:B2012-055)；湖南省科技厅一般项目(No:2014TT2025)；湖南省医药卫生科研计划项目( No:B2013-035) （ No:B2012-055）
