中南医学科学杂志2016，Vol.44Issue(3)：267-270,4.DOI:10.15972/j.cnki.43-1509/r.2016.03.007
miR-185与P KM2在胃癌中的表达及临床意义
Expression of miR-185 and PKM2 in Gastric Cancer and its Clinical Significance
摘要
Abstract
Objective To investigate the expression of miR-185 and PKM2 in gastric cancer and its clinical signifi-cance. Methods In situ hybridization and immunohistochemistry were used to detect the expression of miR-185 and PKM2 in gastric cancer and normal stomach tumour specimens,respectively.The correlations among the miR-185,PKM2, and clinicopathological parameters of gastric cancer patients were analyzed. Results In situ hybridization showed that the positive rate of miR-185 was 38.10% in gastric cancer,and 65.38% in normal stomach tumour specimens,the difference was statistically significant (χ2=14.36,P=0.000).Immunohistochemistry showed that the positive rate of PKM2 was 63. 49%in gastric cancer,and 43.59% in normal stomach tumour specimens,the difference was statistically significant (χ2=7.74, P=0.006).The correlation analysis indicated that the expression of miR-185 had a negative correlation with that of PKM2 in gastric cancer(P<0.01).The positive rate of miR-185 inⅢ~Ⅳstage gastric cancer was strongly lower than inⅠ~Ⅱstage ( P=0.001) ,the expression of miR-185 was also lower with lymph node metastasis than without lymph node metastasis in gastric carcinoma(P=0.003).The positive rate of PKM2 inⅢ~Ⅳ stage gastric cancer was strongly higher than inⅠ~Ⅱstage( P=0.002) ,the expression of PKM2 was also higher with lymph node metastasis than without lymph node metastasis in gastric carcinoma(P=0.006).However,there were no significant difference between different age,gender,histological grade as well as T stage group (P>0.05). Conclusion The low expression of miR-185 and the high expression of the PKM2 protein may be important biological markers for malignant transformation in gastric cancer.关键词
胃癌/miR-185/PKM2/临床意义Key words
gastric cancer/miR-185/PKM2/clinical significance分类
医药卫生引用本文复制引用
石宇雄,郭智维,缪莉,谭志琴..miR-185与P KM2在胃癌中的表达及临床意义[J].中南医学科学杂志,2016,44(3):267-270,4.基金项目
湖南省卫生厅课题( B2014-067) （ B2014-067）
