中南医学科学杂志2016，Vol.44Issue(3)：271-274,289,5.DOI:10.15972/j.cnki.43-1509/r.2016.03.008
miR-200b靶向VEGF抑制肺癌细胞侵袭
miR-200b Promotes Non-small Cell Lung Cancer Cells Invasion by Targeting VEGF
摘要
Abstract
Objective To investigate whether miR-200b promotes cell invasion of non-small cell lung cancer cells by targeting VEGF,thus to reveal molecular mechanism that miR-200b functions as a tumor suppressor in non-small cell lung cancer. Methods The expression of miR-200b in non-small cell lung cancer and normal lung tissues was detected by qRT-PCR.Non-small cell lung cancer cell line A549 were divided into miR-200b mimics group,miR-200b inhibitors group,scramble group, VEGF-siRNA group as well as control-siRNA group. A549 cells were transfected with miR-200b mimics,scramble,VEGF-siRNA,control-siRNA,respectively.The expressions of VEGF protein in A549 cells was detected by Western blotting.The invasion ability of A549 cells were evaluated by Transwell invasion assays. Results qRT-PCR showed that miR-200b was significantly down-regulated in non-small cell lung cancer tissues (0.704±0.053) than normal lung tissues (1.582±0.071).Western blot showed that the level of VEGF protein was inhibited by restored miR-200b or knock-down VEGF in A549 cells.After transfection of miR-200b mimics or knock-down VEGF for 36 h,Transwell invasion assay showed that the number of cells through the basement membrane was(127±6),(136±8),compared with the control group(189±14),it can significantly suppress the invasion of A549 cell (P<0.01).However,miR-200b inhibitors could an-tagonize the role of VEGF-siRNA in A549 cells. Conclusion miR-200b suppresses cell invasion by targeting VEGF in non-small cell lung cancer.关键词
miR-200b/非小细胞肺癌/血管内皮生长因子/侵袭Key words
MiR-200b/non-small cell lung cancer/VEGF/invasion分类
医药卫生引用本文复制引用
罗卫民,罗湘玉,郭家龙,林称意,张军..miR-200b靶向VEGF抑制肺癌细胞侵袭[J].中南医学科学杂志,2016,44(3):271-274,289,5.基金项目
湖北省教育厅科学研究计划指导性项目( B2015477) （ B2015477）
