日用化学品科学2016，Vol.39Issue(7)：25-28,4.DOI:10.13222/j.cnki.dc.2016.07.006
HPLC法测定拳参中绿原酸和 没食子酸含量
Determination of chlorogenic acid and gallic acid in Polygonum bistorta L by HPLC
钱程 1陈玉娥 1黄亮 1张利萍1
作者信息
- 1. 广州立白企业集团有限公司 创新研发中心，广东 广州510170
- 折叠
摘要
Abstract
A method for simultaneous determination of gallic acid and chlorogenic acid in polygonum bistorta L by HPLC-DAD was developed. A SB-C18 column (4.6 mm×0.25 mm,5 µm) was used with a mobile phase of methanol mixed with 0.2%phosphoric acid aqueous solution The lfow rate was 1.0 mL/min. The wavelengths for detection were set at 273 nm and 327 nm and the column temperature was 30 oC. The recoverties of gallic acid and chlorogenic acid were 95%~105%.关键词
绿原酸/没食子酸/拳参提取物/二极管阵列检测器Key words
chlorogenic acid/gallic acid/Polygonum bistorta L/HPLC-DAD分类
化学化工引用本文复制引用
钱程,陈玉娥,黄亮,张利萍..HPLC法测定拳参中绿原酸和 没食子酸含量[J].日用化学品科学,2016,39(7):25-28,4.
