山东农业科学2016，Vol.48Issue(7)：1-9,9.DOI:10.14083/j.issn.1001-4942.2016.07.001
小麦 ent －柯巴基焦磷酸合酶基因 TaCPS1的克隆与荧光定量分析
Cloning and qRT -PCR Analysis of TaCPS1 Gene in Wheat
摘要
Abstract
ent -copalyl diphosphate synthase (CPS)is a key enzyme in the biosynthesis of phytoalex-ins.In this study,the cDNA of TaCPS1 gene which was related to resistance to powdery mildew was identified from the wheat -Thinopyrum alien addition line SN6306 by mRNA differential display.The cDNA had the length of 2 394 bp,which encoded 797 amino acid residues.Bioinformatics analysis indicated that the enco-ded protein which was mainly consisted of hydrophilic amino acids did not contain signal peptide.It was loca-ted in cytoplasm belonging to the Isoprenoid -Biosyn -C1 superfamily.qRT -PCR analysis showed that the expression of TaCPS1 gene in SN6306 was up -regulated about 45 times after induced by E09 for 72 hours, while that in YN15 was basically not induced.The expression of TaCPS1 increased by nearly 15 times and 2.4 times under the induction of JAMe and SA respectively.Thus we hypothesized that TaCPS1 might be in-volved in the regulation pathway of SA and JA in the powdery mildew resistance mechanism.关键词
小麦/ent -柯巴基焦磷酸合酶/TaCPS1/基因克隆/生物信息学分析/荧光定量 PCR/白粉病抗性Key words
Wheat/ent -copalyl diphosphate synthase/TaCPS1/Gene cloning/Bioinformatics analy-sis/Fluorescent quantitative PCR/Powdery mildew resistance分类
农业科技引用本文复制引用
郭庆东,李全权,田秋菊,钱艳丽,许田,杨艳林,王洪刚,封德顺..小麦 ent －柯巴基焦磷酸合酶基因 TaCPS1的克隆与荧光定量分析[J].山东农业科学,2016,48(7):1-9,9.基金项目
国家自然科学基金项目 （）
