山东农业科学2016，Vol.48Issue(7)：26-31,6.DOI:10.14083/j.issn.1001-4942.2016.07.005
萝卜隐性核雄性不育系136A 的选育及其育性研究
Breeding of Radish Recessive Nuclear Male Sterile Line 136A and Its Fertility Study
摘要
Abstract
136A,a new type of radish nuclear male sterile material,was found from the natural popula-tion.Through crossing with fertile plants in 178 hybrid combinations,136B was selected,which had the abili-ty to maintain the sterility of 50%.Then the stable and homozygous male sterile line 136AB was bred through several generations.The field genetic experiment showed that the sterile trait of 136A was stable,and ex-pressed the nuclear sterile genetic feature which was controlled by a single recessive gene.The cytological ob-servation indicated that the pollen of 136A was thoroughly abortive,the pollen development began abnormal from the tetrad stage,and pollen abortion was mainly induced by the rupture of pollen wall.The PCR results showed that 136A did not contain orf138 gene,which was the major gene of Ogura male sterile type.The mi-tochondrial and chloroplast DNA sequencing results also showed that 136A had the normal fertile cytoplasm.关键词
萝卜/核雄性不育/136A/保持系Key words
Radish/Genetic male sterility/136A/Maintainer line分类
农业科技引用本文复制引用
白静,段乃彬,谢坤,王俊峰,王效睦..萝卜隐性核雄性不育系136A 的选育及其育性研究[J].山东农业科学,2016,48(7):26-31,6.基金项目
山东省自然科学基金项目“萝卜新型不育材料 orf138基因的定位与遗传规律的研究”（ZR2015YL054）；山东省农业良种工程项目“农业生物资源创新利用”（2013lz017）；山东省农业科学院青年基金项目 （）
