山东医药2016，Vol.56Issue(25)：5-7,3.DOI:10.3969/j.issn.1002-266X.2016.25.002
人类肝癌细胞 HepG2、Bel-7402和 Li-7植入裸鼠体内后微量蛋白表达分析
Expression of microproteins in nude mice injected with hepatoma ceII Iines of HepG2,BeI-7402 and Li-7
摘要
Abstract
Objective To investigate the serum proteinogram of hepatoma cell lines (HepG2,Bel-7402 and Li-7) which grow in the nude mice in order to find the microprotein markers which are associated with early diagnosis of hepato-ma.Methods We classified 40 BALB/cA-nu nude mice into 4 groups randomly and marked them as HepG2 group,Li-7 group,Bel-7402 group and control group,respectively.Three types of hepatoma cell lines (HepG2,Bel-7402 and Li-7) were cultured and the cells were prepared into cell suspension (1.5 ×107 /ml),respectively.We injected the cell suspen-sion into the relevant groups with 0.2 mL in every mouse and the control group was injected with RPMI1640 medium. These nude mice were observed for 2 months and we got their blood on the 20th,40th,60th day by eyeball extirpating.We detected the proteinogram using surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS)and then,we analyzed the data by Ciphergen ProteinChip 3.0 and Ciphergen Biomaker Wizard 3.1 and SPSS 16.0 to screen the differentiated proteins.Results After the mice in HepG2 group were injected with cell line HepG2,there were 29 differentiated proteins.Among them,8 proteins changed significantly and 5 proteins were up-regulated and 3 were down-regulated.After the mice in Li-7 group were injected with cell line Li-7,there were 12 differentiated proteins.Among them,7 proteins enhanced,and 5 proteins weakened.After the mice in Bel-7402 group were injected with cell line Bel-7402,there were 5 differentiated proteins.Among them,4 proteins enhanced and 1 protein weakened.Conclusions Dif-ferent subtypes of hepatoma cell lines injecting into nude mice can express different microproteins in the serum of mice. These proteins could be identified and detected to be promising markers of different subtypes of hepatoma for the early diag- <br> nosis.关键词
肝肿瘤/表面增强激光解吸电离飞行时间质谱仪/蛋白质组学/差异性蛋白/质荷比/裸鼠Key words
liver neoplasms/surface enhanced laser desorption/ionization time of flight mass spectrometry/proteomics/differential protein/mass-to-charge ration/nude mice分类
医药卫生引用本文复制引用
曾小峻,陶华林,汪碧琼,于卉..人类肝癌细胞 HepG2、Bel-7402和 Li-7植入裸鼠体内后微量蛋白表达分析[J].山东医药,2016,56(25):5-7,3.基金项目
四川省科技支撑计划项目（2013JY0102）。 （）
