山东医药2016，Vol.56Issue(25)：20-23,4.DOI:10.3969/j.issn.1002-266X.2016.25.006
BCRP 过表达对乳腺癌细胞增殖和凋亡的影响
InfIuence of BCRP overexpression on proIiferation and apoptosis of human breast cancer ceIIs
摘要
Abstract
Objective To investigate the effect of breast cancer resistance protein (BCRP)overexpression on cell proliferation and apoptosis of breast cancer cells MDA-MB-435S.Methods MDA-MB-435S cells in the logarithmic phase were randomly divided into three groups:the positive transfection group,in which the eukaryotic expression plasmid pcD-NA3.1 /BCRP was identified by restriction analysis and sequencing and then was transfected into MDA-MB-435S cells,the positive control group,in which the cells were transfected with 2 μg pcDNA3.1 empty plasmid at the same time,and the blank control group,in which the cells were cultured with 2 mL nutrient solution.The mRNA and protein expression of BCRP was determined by RT-PCR and Western blotting,respectively.After 48-hour transfection,7 mitomycin (MMC) concentration groups (0.01,0.10,2.50,5.00,10.00,20.00,40.00 μmol/L)were set up in each group,each con-centration group had 3 complex holes which were added with 10 μL MMC.Cell counting kit-8 (CCK-8)and flow cytometry were used to determine the influence of BCRP on cell proliferation and apoptosis of MDA-MB-435S cells,respectively.Re-sults Restriction analysis and sequencing proved that recombinant plasmid pcDNA3.1 /BCRP was constructed correctly. Both the expression of BCRP mRNA and protein was up-regulated in MDA-MB-435S cells after transfection 24,48 or 72 h in the positive transfection group (P <0.05).No significant difference was found between the positive transfection group and the negative control group (P >0.05).After MMC treatment of 24 hours,CCK-8 showed that the cell survival rate in the transfection group was significantly higher than that of the negative control group (P <0.05).Conclusion BCRP overexpression promotes the proliferation of MDA-MB-435S cells,but has no significant effect on apoptosis.关键词
乳腺癌/乳腺癌耐药蛋白/细胞增殖/细胞凋亡Key words
breast carcinoma/breast cancer resistance protein/cell proliferation/apoptosis分类
医药卫生引用本文复制引用
于兆进,余涧坤,于丽凤,魏敏杰,赵琳..BCRP 过表达对乳腺癌细胞增殖和凋亡的影响[J].山东医药,2016,56(25):20-23,4.基金项目
国家自然科学基金资助项目（81573462）；辽宁省高等学校优秀人才支持计划项目（LJQ2015118）。 （）
