山东医药2016，Vol.56Issue(26)：6-9,4.DOI:10.3969/j.issn.1002-266X.2016.26.002
他莫昔芬对乳腺癌 MCF-7细胞侵袭能力、MMP-9活性和表达的影响及机制
Effects of tamoxifen on invasion,activity and expression of MMP-9 in breast cancer MCF-7 ceIIs
摘要
Abstract
Objective To explore the effects of tamoxifen (TAM)on the invasion,activity and expression of matrix met-alloproteinase-9 (MMP-9)in breast cancer MCF-7 cells,meanwhile to discuss the role of inhibiting G-protein-coupled receptor 30 (GPR30)in these effects.Methods MCF-7 cells in the logarithmic phase were pre-cultured for 24 h in phenol red (PR)-free medium without serum to remove endogenous estrogen before the indicated treatments.MCF-7 cells were seeded in the six-well plates and incubated over night to let them adhere on the plate.The control group was continued to cultivate in PR-free me-dium without serum for 24 h.The TAMgroup was treated with 1 μmol/L TAMin PR-free medium without serum for 24 h.And the TAM+G15 group was pretreated with G15 (1 μmol/L)for 30 min before treatment with TAM(1 μmol/L)for 24 h in PR-free medium without serum.After treatment,cell invasion was detected by Transwell assay.The activity of MMP-9 in culture me-dium was tested by Gelatin zymography assay.The MMP-9 protein expression was analyzed by Western blotting.The mRNA ex-pression of MMP-9 was tested by real-time RT-PCR.Results Compared with control group,the cell invasion was increased, the protein and mRNA expression level of MMP-9 was up-regulated,and the activity of MMP-9 in MCF-7 cells was increased in the TAMgroup (all P <0.05).Furthermore,compared with the TAMgroup,the above indexes of the TAM+G15 group were all decreased (all P <0.05).Conclusions TAMpromotes the cell invasion ability and up-regulates the activity and expression of MMP-9.Meanwhile,the effects may be suppressed by G15 pretreatment.关键词
他莫昔芬/乳腺癌/基质金属蛋白酶9/G蛋白偶联受体30Key words
tamoxifen/breast carcinoma/G-protein-coupled receptor 30/matrix metalloproteinase-9分类
医药卫生引用本文复制引用
陈妍,王婧,徐旖旎,洪端阳,潘迪,沈祥春..他莫昔芬对乳腺癌 MCF-7细胞侵袭能力、MMP-9活性和表达的影响及机制[J].山东医药,2016,56(26):6-9,4.基金项目
国家自然科学基金资助项目（81302804）；贵州省科学技术基金资助项目（黔科合 J 字20142007号）；贵州省高校优秀科技创新人才支持计划项目（黔教合 KY 字2015492）；贵州省中医药管理局中医药、民族医药科学技术研究课题（20141001）。 （）
