山东医药2016，Vol.56Issue(26)：10-12,3.DOI:10.3969/j.issn.1002-266X.2016.26.003
雷米普利对慢性心衰大鼠肾间质纤维化及PDGF-B、CTGF表达的影响
Effects of ramipriI on renaI interstitiaI fibrosis,expression of PDGF-B and CTGF in rats with chronic heart faiIure
摘要
Abstract
Objective To observe the effects of ramipril on the renal interstitial fibrosis,expression of platelet derived growth factor (PDGF-B)and connective tissue growth factor (CTGF)in the renal tissues of rats with chronic heart failure. Methods The 30 male Wistar rats were randomly divided into the Sham group (group A,10 rats),model group (group B,10 rats)and the ramipril group (group C,10 rats).The chronic heart failure model was established by the renal upper abdominal aorta coarctation.After the model was successfully established,the rats in the group C were administrated with 1 mg/(kg·d)ramipril for 4 weeks,while rats in groups A and B were administrated with normal saline for 4 weeks.The degree of renal fibrosis was detected under light microscope by HE and Masson staining.The protein and mRNA expression levels of PDGF-B and CTGF were respectively detected by immunohistochemistry and RT-PCR.Results Compared with group A,the renal fibrosis was significantly found in the groups B and C,and group B was more severe than the group C. The protein and mRNA expression levels of PDGF-B and CTGF were significantly higher in the groups B and C than that those of group A,and moreover,group B was higher than group C (all P <0.01).Conclusion Ramipril can effectively decrease the renal interstitial fibrosis,expression levels of PDGF-B and CTGF in rats with chronic heart failure.关键词
心力衰竭/雷米普利/肾脏纤维化/血小板源性生长因子/结缔组织生长因子Key words
heart failure/ramipril/renal fibrosis/platelet-derived growth factor/connective tissue growth factor分类
医药卫生引用本文复制引用
周静,杨晓鸥,张梦云,李秀华,徐婧,谢亚芹..雷米普利对慢性心衰大鼠肾间质纤维化及PDGF-B、CTGF表达的影响[J].山东医药,2016,56(26):10-12,3.基金项目
河北省卫计委科研基金资助项目（20110180）。 （）
