山东医药2016，Vol.56Issue(27)：1-4,4.DOI:10.3969/j.issn.1002-266X.2016.27.001
miR-181c在胶质母细胞瘤组织中的表达变化及其对肿瘤细胞侵袭、迁移能力的影响
Expression changes of miR-181c in glioblastoma and its effects on invasion and migration of tumor cells
摘要
Abstract
Objective To observe the expression changes of microRNA181c (miR-181c) in glioblastoma (GBM) and its effects on invasion and migration of tumor cells.Methods real-time PCR was used to detect the miR-181c expression in 41 GBM tissues (GBM group) and 15 normal brain tissues (control group).GBM cell line T98G was divided into four groups:groups A, B, C and D.In the groups A, B and C, the T98G was transfected with miR-181c with overexpression, control miRNA and miR-181c, respectively, but the group D without any treatment.After transfection 48 h, the invasion and migration in each group was investigated.Results Compared with the control group, the miR-181c expression was down-regulated in the GBM group, P<0.05.After transfection 48 h, the invasive cells in the groups B, C and D were more than those of the group A, P<0.05.Moreover, cell relative migration distance in the groups B, C and D was also longer than that of the group A, P<0.05.Conclusion The miR-181c is lowly expressed in GBM tissues, and the low miR-181c expression is closely related with GBM cell invasion and migration.关键词
微小RNA181c/胶质瘤/胶质母细胞瘤/细胞迁移/细胞侵袭Key words
microRNA181c/glioma/glioblastoma/cell migration/cell invasion分类
医药卫生引用本文复制引用
田伟,张娟,王利娜,张泰民,贾万良,高岩,马文群..miR-181c在胶质母细胞瘤组织中的表达变化及其对肿瘤细胞侵袭、迁移能力的影响[J].山东医药,2016,56(27):1-4,4.基金项目
河北省卫生厅青年基金资助项目（20150036）。 （）
