山东医药2016，Vol.56Issue(27)：12-15,4.DOI:10.3969/j.issn.1002-266X.2016.27.004
舒心安神膏灌胃对大鼠自主活动的影响及联合戊巴比妥钠腹腔注射对小鼠睡眠和肌肉协调力的影响
Effect of Shuxin Anshen paste on rat′s autonomic activity and the effect of Shuxin Anshen paste and sodium pentobarbital on mouse′s sleep and motor coordination
摘要
Abstract
Objective To observe the effect of Shuxin Anshen paste on rat′sautonomic activities by intragastric admin-istration and the effect of Shuxin Anshen paste combined with sodium pentobarbital on mouse′s sleep and motor coordination by intraperitoneal injection.Methods Fifty SPF SD rats were randomly divided into groups A, B, C, D and E, each group had 10 rats.Groups A, B, C were separately given 1 mL/100 g Shuxin Anshen paste at the concentration of 0.28 g/mL, 0.84 g/mL and 1.40 g/mL by intragastric administration once a day for 5 days.Group D was given 1 mL/100 g dia-zepam by intragastric administration on the 5th day.Group E was given 1 mL/100 g pure water as the control group.Sixty minutes after the last drug administration, all rats were put in the spontaneous activity box.The times of standing, groo-ming, central grilling and total crossing in 5 minutes were recorded.Fifty NIH mice were randomly assigned into low dosage group, medium dosage group, high dosage group, diazepam group and blank group, each group had 10 mice.Low dosage group, medium dosage group and high dosage group were separately given 0.2 mL/10 g Shuxin Anshen paste at the concen-tration of 0.205, 0.615 and 1.025 g/mL by intragastric administration once a day for 5 days.Diazepam group and control group were separately given 4 mg/kg diazepam and 1 mL/100 g pure water by intragastric administration on the 5th day. Sixty minutes after the last drug administration, each group was separately given pentobarbital sodium 45 ( the up threshold dose) and 40 ( the under threshold dose) mg/kg by intraperitoneal injection.All groups′sleep latency and sleep time was measured and recorded.Fifty NIH mice were randomly assigned into groups 1, 2, 3, 4 and 5.Groups 1, 2 and 3 were giv-en 0.2 mL/10 g Shuxin Anshen paste at the concentration of 0.205, 0.615 and 1.025 g/mL by intragastric administration once a day for 5 day.Groups 4 and 5 were separately given 4 mg/kg diazepam and 0.2 mL/10 g pure water by intragastric administration on the 5th day.Sixty minutes after the last drug administration, each group was put on the rolling-bar device and was observed for 5 minutes.Falling latency and total falling times in 5 minutes were recorded.Results In the auto-nomic activity test, the times of standing and the central grilling of groups A,B,C and D were significantly less than that of group E (all P<0.05).In the sedative and hypnotic experiment using Shuxin Anshen paste and pentobarbital sodium (the up threshold dose) , the sleep latency among low dosage group, medium dosage group, high dosage group, diazepam group and control group was not statistically significant, but the sleep time of these group was significantly higher than that of the control group (all P<0.05).In the experiment of promoting the hypnosis in mice by Shuxin Anshen paste and pentobarbi-tal sodium with the under threshold dose, the sleep latency of low dosage group, medium dosage group, high dosage group, diazepam group and control group was (3.89 ±3.02), (3.20 ±4.32), (2.80 ±2.14), (5.30 ±2.75) and (15 ±0) min.The sleep time of these groups was (36.00 ±4.53), (38.00 ±5.90), (40.29 ±7.36), (36.30 ±10.32) and 0 min.The falling latency of group 4 was significantly less than that of the other groups, and the falling time in 5 minutes of group 4 was significantly higher than that of the other groups (all P<0.05).Conclusions Shuxin Anshen paste can sup-press the rat′s autonomic activity.The synergism of Shuxin Anshen paste and pentobarbital sodium can promote the mouse′s sleep and have little influence on the muscle coordination.关键词
舒心安神膏/大鼠/小鼠/镇静/催眠/肌肉协调运动能力Key words
Shuxin Anshen paste/rats/mice/sedation/hypnosis/muscle coordination分类
医药卫生引用本文复制引用
徐福平,梁皓越,林立宇,杨志敏..舒心安神膏灌胃对大鼠自主活动的影响及联合戊巴比妥钠腹腔注射对小鼠睡眠和肌肉协调力的影响[J].山东医药,2016,56(27):12-15,4.基金项目
国家自然科学基金青年基金资助项目（81503515）；广东省自然科学基金资助项目（2015A030310255）；广东省科技厅计划项目（2014A020221088）。 （）
