山东医药2016，Vol.56Issue(27)：20-23,4.DOI:10.3969/j.issn.1002-266X.2016.27.006
呼吸道合胞体病毒F蛋白二级结构分析及其B细胞表位预测
Secondary structure and prediction of B cell epitope of F protein in respiratory syncytial virus
摘要
Abstract
Objective To analyze the secondary structure and predict its B cell epitope of F protein in respiratory syncytial virus ( RSV) .Methods We analyzed the secondary structure of RSV F protein and obtained the sequence and location about the signal peptide in RSV F protein.Based on the homology modeling method, we predicted the potential conformational B epitopes and linear B epitopes in RSV F protein.Results Among the 574 amino acids of RSV F protein, the major amino acids were serine (10.45%), leucine (10.28), asparagine (8.71%) and threonine (8.71%).There might be 7 protein binding sites, 6 helical structure generation regions and 2β-plated sheets in F protein.The sites 1-25 of N-terminus in F protein were signaling peptides.The head of the F protein was mainly composed byβ-plated sheets, ran-dom coils and turns, while the tail was mainly composed by 2 long α-helixes.Eight linear B epitopes and four conforma-tional B epitopes were predicted in F protein.Conclusions Serine and leucine occupy the maximum ratio of amino acids encoding F protein.There might be seven protein binding sites, six helical structure generation regions, two majorβ-plated sheets in F protein, and RSV F protein may contain four conformational B epitopes and eight linear B epitopes.关键词
呼吸道合胞体病毒/F蛋白/蛋白二级结构/B细胞表位Key words
respiratory syncytial virus/F protein/secondary structure of protein/B cell epitope分类
医药卫生引用本文复制引用
易高,潘嘉宇,林纯意,刘兆宇..呼吸道合胞体病毒F蛋白二级结构分析及其B细胞表位预测[J].山东医药,2016,56(27):20-23,4.基金项目
国家自然科学基金青年科学基金项目（81400013）；广东省科技计划自助项目（2014A020212329）。 （）
