山东医药2016，Vol.56Issue(28)：5-8,4.DOI:10.3969/j.issn.1002-266X.2016.28.002
玫瑰花蕾萃取物对嗅球毁损大鼠梨形皮质神经元损伤修复的影响及机制
Effect of rosebud extracts on piriform cortical neuronal damage and repair in olfactory bulb damaged rats and its mechanism
摘要
Abstract
Objective To investigate the effect of rosebud extracts on piriform cortical neuronal damage and repair in olfactory bulb damaged rats and its mechanism.Methods Healthy adult male SD rats were randomly divided into three groups, namely the control group (n=10), model group (n=40) and rosebud group (n=10).Rats in the control group were not treated.Using probes agitation to damage the olfactory bulb of rats in the model group and rosebud group.One day after the damage of the olfactory bulb, rats in the rosebud group were prepared to smell rosebud extracts, each time for 1 h, twice a day and for 14 d.The brain tissues were collected from rats in the model group after the damage of the olfactory bulb 24 h, 72 h, 7 d and 14 d ( each time, ten rats) as well as the rosebud group 14 d after the damage.After convention-al section, we used Nissl staining to detect the piriform cortical neuronal damage and repair.Immunohistochemical staining was carried out to detect the Glu and GABA expression of piriform cortex.Results The Nissl body gray value of the con-trol group was 87.32 ±3.14, and they were respectively 101.76 ±4.52, 110.76 ±2.78, 98.35 ±2.69 and 94.26 ±3.01 after olfactory bulb damage of 24 h, 72 h, 7 d and 14 d.The rosebud group was 88.47 ±4.33, and the Nissl body gray value in 72 h after olfactory bulb damage was higher than that of the control group (all P<0.05).Compared with the con-trol group, the Glu expression gray value of piriform cortex at 72 h and 7 d after olfactory bulb damage was decreased, but the GABA expression gray value was increased in the model group.The Glu expression gray value of piriform cortex was in-creased and the GABA expression gray value was decreased in the rosebud group as compared with that of the model group at 72 h and 7 d after olfactory bulb damage.Significant difference was found between these two groups (all P<0.05). Conclusion Rosebud extracts play an important role in promoting the piriform cortical neuronal damage and repair in ol-factory bulb damaged rats, and its mechanism may be associated with the inhibiting release of Glu, and promoting release of GABA.关键词
玫瑰花蕾萃取物/嗅球毁损/梨形皮质/神经递质/嗅觉通路/神经元再生Key words
rosebud extracts/olfactory bulb damage/piriform cortical/neurotransmitter/olfactory pathway/neuro-genesis分类
医药卫生引用本文复制引用
卞林翠,王敏,贺利敏,徐金勇,李光武..玫瑰花蕾萃取物对嗅球毁损大鼠梨形皮质神经元损伤修复的影响及机制[J].山东医药,2016,56(28):5-8,4.基金项目
国家自然科学基金资助项目（81000589）。 （）
