山西中医学院学报2016，Vol.17Issue(4)：25-27,35,4.
地肤病毒病的病原分析
Pathogenic analysis of kochia scoparia virus disease
弓强 1张春明 2牛颜冰3
作者信息
- 1. 山西中医学院，山西晋中030619
- 2. 山西省兴县综合检验检测中心，山西兴县033600
- 3. 山西农业大学，山西太谷，030801
- 折叠
摘要
Abstract
Objective:Analyze the pathogeny on the Kochia scoparia virus disease. Method:The Kochia scoparia virus was identified,the virus double-stranded RNA (dsRNA)was extracted,and the single cDNA was synthesized using the multiplex PCR amplification method,and the target sequence was analyzed. Result:The Mosaic symptoms of K. scoparia were caused by the infection of Broad bean wilt virus 2 (Broad bean wilt virus 2,BBWV2). Sequence homology analysis revealed that the partial virus sequence from Isolate-DF of K. scopria showed 79.9%~88.0% homology with other 15 reported BBWV sequences. Conclusion:The results of nucleotides homology analyzing firstly confirmed that the Mosaic virus disease of medicinal plant K.scopria was caused by BBWV2 in china.关键词
地肤/生物学检测/多重PCR扩增/蚕豆萎蔫病毒2号Key words
Kochia scopria/Biological detection/the multiplex PCR amplification/Broad bean wilt virus 2分类
医药卫生引用本文复制引用
弓强,张春明,牛颜冰..地肤病毒病的病原分析[J].山西中医学院学报,2016,17(4):25-27,35,4.
