色谱2016，Vol.34Issue(8)：745-751,7.DOI:10.3724/SP.J.1123.2016.05027
聚烯丙基氯点击反应接合葡萄糖纳米磁球的制备与糖肽富集
Preparation and glycopeptide enrichment application of magnetic nanoparticles functionized by poly-(allyl chloride)-glucose based on click reaction
摘要
Abstract
The abundance of glycoproteins and the glycans attached are usually low in body. In order to analyze them accurately and eliminate the interference of non-glycoproteins and non-glycopeptides,methods of enrichment are needed. A kind of novel magnetic nanoparticles based on click reaction and multiple-functional-site poly-( allyl chloride)structure is designed and prepared. The related hydrophilic enrichment method for glycopeptide is developed. Totally 32 glycopeptide signals were detected from human immunoglobulin G trypsin enzymolysis under the optimized conditions. The method can reach a limit of detection of 10 fmol on human immu-noglobulin G trypsin enzymolysis,with an adsorption capacity of 100 mg of 1 g particles.关键词
点击反应/磁性纳米富集材料/糖肽/人血清免疫球蛋白 GKey words
click reaction/magnetic enrichment nanomaterials/glycopeptide/human immunoglobulin G分类
化学化工引用本文复制引用
高小迪,张维冰..聚烯丙基氯点击反应接合葡萄糖纳米磁球的制备与糖肽富集[J].色谱,2016,34(8):745-751,7.基金项目
国家自然科学基金项目（21235005）；国家重大科学仪器设备开发专项（2012YQ120044）．@@@@Natural Science Foundation of China（ No．21235005）；National Major Scientific Instruments and Equipment Development Projects of China（ No．2012YQ120044）． （）
