水生生物学报2016，Vol.40Issue(4)：663-671,9.DOI:10.7541/2016.89
饲料中添加溶菌酶对吉富罗非鱼生长、免疫-抗氧化功能及血清抗菌性能的影响
EFFECTS OF DIETARY LYSOZYME SUPPLEMENTATION ON GROWTH PERFORMANCE, IMMUNE-ANTIOXIDANT CAPACITY AND SERUM ANTIBACTERIAL PROPERTIES OF GIFT TILAPIA (OREOCHROMIS NILOTICUS)
摘要
Abstract
To investigate effects of dietary lysozyme supplementation on growth performance, immune-antioxidant ca-pacity and antibacterial properties of GIFT tilapia (Oreochromis niloticus), basal diet or basal diet added with 18, 36, 54, 72 or 90 mg/kg lysozyme were formulated for a 60 days experiment. The results showed that the 54 mg/kg lyso-zyme addition group had the best growth and feed efficiency in which weight gain rate and protein efficiency ratio in-creased and feed conversion ratio decreased significantly compared with the control group (P<0.05). Lysozyme supple-mentation decreased hepatosomatic index with significant difference in 90 mg/kg group compared with the control group (P<0.05). Spleen-somatic index was significantly lower in 36 mg/kg and 54 mg/kg groups compared with that of the control group (P<0.05). Crude protein and ash content in whole body in 54 mg/kg group were significantly higher than that of the control group (P<0.05). Lysozyme supplementation in 54 and 72 mg/kg group increased serum and li-ver superoxide dismutase and catalase activity but significantly decreased malondialdehyde content compared with the control group (P<0.05). Liver lysozyme activity in 54 and 72 mg/kg group was significantly higher than that of the con-trol group, while serum lysozyme activity in groups except L90 group was significantly lower than that of the control group (P<0.05). Serum antibacterial test showed more resistance to Escherichia coli, Staphylococcus aureus, Aeromo-nas hydrophila, Vibrio alginolyticus in 54 and 72 mg/kg group compared with the control group but more sensitive to Bacillus subtilis with a 34.71%and 42.21%reduction, respectively. These results indicated that diet lysozyme supple-mentation (such as 54 mg/kg) can improve GIFT tilapia growth performance by regulating immune-antioxidant capa-city of tilapia and improving serum antibacterial properties.关键词
吉富罗非鱼/溶菌酶/生长/免疫-抗氧化功能/抗菌性能Key words
GIFT tilapia/Lysozyme/Growth performance/Immune-antioxidant capacity/Antimicrobial activity分类
农业科技引用本文复制引用
王坛,华雪铭,朱伟星,陈晓明,吴钊,孔纯,焦建刚,何亚丁,王刚..饲料中添加溶菌酶对吉富罗非鱼生长、免疫-抗氧化功能及血清抗菌性能的影响[J].水生生物学报,2016,40(4):663-671,9.基金项目
广东省教育部产学研结合项目(2012B091100372) （2012B091100372）
现代农业产业技术体系专项资金(CARS-49-04B) （CARS-49-04B）
水产动物遗传育种中心上海市协同创新中心(ZF1206)资助[Supported by Ministry of Education of Guangdong Province University-industry Cooperation Project (2012B091100372) （ZF1206）
China Agriculture Research System (CARS-49-04B) （CARS-49-04B）
Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding (ZF1206)] （ZF1206）
