水生生物学报2016，Vol.40Issue(4)：736-743,8.DOI:10.7541/2016.97
谷氨酰胺对半滑舌鳎稚鱼非特异性免疫相关酶活力和低氧应激后HIF-1α表达的影响
EFFECTS OF DIETARY GLUTAMINE ON ACTIVITIES OF NON-SPECIFIC IMMUNE RELATED ENZYMES AND HIF-1α EXPRESSION AFTER HYPOXIA OF HALF-SMOOTH TONGUE SOLE POST LARVAE
摘要
Abstract
To investigate effects of dietary glutamine on activities of non-specific immune related enzymes and anti-hypoxia stress capacity of half-smooth tongue sole (Cynoglossus semilaevis Günther) post larvae, four isonitrogenous and isolipidic experimental diets supplemented with 0, 0.5%, 1.0%and 2.0%gluta-mine (with dietary free glutamine estimated to be about 0.03%, 0.46%, 0.91%and 1.73%) were formulated and fed 5 times per day to C. semilaevis post larvae (35 days after hatching, 10.64±0.86 mg dry weight) for 30 days before hypoxia stress test. Results revealted that the 0.5%glutamine supplemented diet significantly increased activities of lysozyme in fish whole body compared to the control group (P<0.05), but different glutamine level did not impact the activities of total nitric oxide synthase and in-ducible nitric oxide synthase in fish whole body (P>0.05). C. semilaevis HIF-1αgene were cloned and 749 bp partial cDNA sequence were obtained, which shared high identities with many other fish species. Quantitative Real-time PCR showed that glutamine supplementation did not regulate the expressions of HIF-1αin fish after hypoxia stress (P>0.05). In summary, dietary glutamine could enhacne the activities of LZM and the non-specific immunity of C. semilaevis post larvae.关键词
谷氨酰胺/半滑舌鳎/仔稚鱼/非特异性免疫/HIF-1α/基因克隆及定量Key words
Glutamine/Cynoglossus semilaevis/Post larvae/Non-specific immune/HIF1-α/Gene cloning and expression分类
农业科技引用本文复制引用
刘经纬,麦康森,徐玮,张彦娇,周慧慧,艾庆辉..谷氨酰胺对半滑舌鳎稚鱼非特异性免疫相关酶活力和低氧应激后HIF-1α表达的影响[J].水生生物学报,2016,40(4):736-743,8.基金项目
国家鲆鲽类产业技术体系(编号:CARS50-G08)资助[Supported by the National Flatfish Industry System Construction Programme (No. CARS50-G08)] （编号:CARS50-G08）
