水生生物学报2016，Vol.40Issue(4)：744-751,8.DOI:10.7541/2016.98
饲料缬氨酸水平对军曹鱼鱼体脂肪含量、血浆生化指标和肝脏脂肪代谢基因表达的影响
THE EFFECTS OF VALINE LEVEL ON PLASMA BIOCHEMICAL INDEXES, LIPID CONTENT AND GENE EXPRESSION INVOLVED IN LIPID METABOLISM IN COBIA (RACHYCENTRONCANADUM)
摘要
Abstract
The present study was conducted to investigate the effects of dietary valine on plasma biochemical indexes, lipid content and gene expression involved in lipid metabolism in cobia (Rachycentron canadum). Fish [mean initial weight, (40.9±0.8) g] were fed with soybean meal based on diets with graded levels of valine (1.26%, 2.21%and 2.62%) for 10 weeks. Results showed that lipid content of the whole body and muscle of fish fed the diet with deficient valine (1.26%) was significantly lower than that fish fed the moderate (2.21%) and excess (3.23%) valine treatment groups (P<0.05). Plasma total protein (TP) fish increased significantly as dietary valine increased from 1.26%to 2.21%(P<0.05), and kept relatively constant when dietary valine level was above 2.21%(P>0.05). Plasma total cholesterol (TC) and the lipid content of liver increased with dietary valine increasing from 1.26%to 2.21%(P<0.05), but de-creased with higher levels of dietary valine (2.21%to 2.62%) (P>0.05). Hepatic mRNA levels of lipid synthesis related genes (SREBP-1, and FAS) were significantly up-regulated in fish fed the diet with moderate level of valine (2.21%) (P<0.05), while hepatic mRNA transcriptional levels PPARαwere significantly elevated in fish fed the diet with high level of valine (P<0.05). Overall, results of this study suggested that valine deficiency could decrease lipid content and inhibit expressions of some lipid synthesis related genes of cobia. This may contribute to understanding the mecha-nisms related to the physiological effects of dietary valine in cobia.关键词
军曹鱼/缬氨酸/脂肪含量/生化指标/脂肪代谢Key words
Cobia/Valine/Lipid content/Plasma biochemical indexes/Lipid metabolism分类
农业科技引用本文复制引用
王震,徐玮,麦康森,路凯,刘迎隆,艾庆辉..饲料缬氨酸水平对军曹鱼鱼体脂肪含量、血浆生化指标和肝脏脂肪代谢基因表达的影响[J].水生生物学报,2016,40(4):744-751,8.基金项目
国家公益性行业(农业)科研专项(201003020,200903029)资助[Supported by the Ministry of Agriculture of the People’s Republic of China (No.201003020,200903029)] （农业）
