水生生物学报2016，Vol.40Issue(4)：752-757,6.DOI:10.7541/2016.99
大黄鱼CCTα基因的克隆及其表达量随稚鱼生长发育的变化
MOLECULAR CLONING AND GENETIC ONTOGENY OF CCTαDURING THE DEVELOPMENT OF LARGE YELLOW CROAKER LARVAE (LARIMICHTHYSCROCEA)
摘要
Abstract
In the present study, the full length of CCTα(CTP:choline phosphate cytidylyltranferase) cDNA from large yellow croaker (Larimichthys crocea) was obtained by homology-based cloning and rapid amplification of cDNA ends (RACE) techniques. Sequence analysis showed that the full length of CCTα cDNA (GenBank accession No. KF006240.1) was 2419 bp, which consisted of 1107 bp open-reading frame (ORF) encoding 369 amino acids, a 273 bp 5'-untranslated region (5'UTR) and a 1010 bp 3'-untranslated region (3'UTR). Phylogenetic tree analysis showed that the CCTαgene of large yellow croaker had a closer relationship with Takifugu rubripes than others. The expression of CCTαincreased significantly at first, and then decreased, and peaked at 15 day after hatching. The genetic ontogeny of CCTαwas related to the development of large yellow croaker digestive system.关键词
大黄鱼/磷脂/合成代谢/个体发育/磷脂酰胆碱胞苷酰基转移酶Key words
Large yellow croaker/Phospholipid/Anabolism/Development/Choline phosphate cytidylyltranferase (CTP)分类
生物科学引用本文复制引用
冯硕恒,蔡佐楠,麦康森,艾庆辉..大黄鱼CCTα基因的克隆及其表达量随稚鱼生长发育的变化[J].水生生物学报,2016,40(4):752-757,6.基金项目
国家自然科学基金“大黄鱼仔稚鱼磷脂营养代谢研究”(31172425)资助[Supported by the National Natural Science Foundation of China“Study on Phospholipids Metabolism of Large Yellow Croaker Larvae”(Grant No.31172425)] （31172425）
