水生生物学报2016，Vol.40Issue(4)：758-766,9.DOI:10.7541/2016.100
氧化鱼油对草鱼肠道黏膜抗氧化应激通路基因表达水平的影响
EFFECTS OF OXIDIZED FISH OIL ON OXIDATIVE STRESS PATHWAYS OF INTESTINAL MUCOSA OF GRASS CARP (CTENOPHARYNGODON IDELLUS)
摘要
Abstract
To evaluate effects of the oxidized fish oil on the antioxidative stress pathways of intestine, grass carp Cteno-pharyngodon idellus were fed oxidized fish oil and normal fish oil for 7d before collecting intestinal mucosa for histo-logy and RNA-seq. The gene differential expression, the gene annotation and IPA gene pathway were analyzed. Severe damage was observed in the intestine of grass carp by oxidized fish oil. The RAN-seq results indicated increased“Keap1-Nrf2-ARE”signal pathway and GSH/GSTs pathway by oxidized fish oil treatment. The heat shock proteins and ubiquitin-proteasome system genes were also significantly up-regulated. The activations of these three anti-oxida-tion pathway systems serve the main components to remove the damaged cells and decompose the harmful proteins, which play the essential roles to protect the intestinal mucosa tissue and its cells and to repair the intestinal mucosa re-sponse to the oxidation injury.关键词
草鱼/肠道黏膜/氧化鱼油/RNA-seq/Nrf2/GSH/GSTs/泛素-蛋白酶体Key words
Grass carp Ctenopharyngodon idellus/Intestinal mucosa/Oxidized fish oil/RNA-seq/Nrf2/GSH/GSTs/Protein ubiquitination分类
农业科技引用本文复制引用
叶元土,蔡春芳,许凡,林秀秀,黄雨薇,董娇娇,张宝彤,萧培珍..氧化鱼油对草鱼肠道黏膜抗氧化应激通路基因表达水平的影响[J].水生生物学报,2016,40(4):758-766,9.基金项目
国家自然基金(31172417)资助[Supported by the National Natural Science Foundation of China (No.31172417)] （31172417）
