水生生物学报2016，Vol.40Issue(4)：767-778,12.DOI:10.7541/2016.101
育肥饲料中植物油混合替代鱼油对中华绒螯蟹雄体脂肪酸代谢相关基因表达的影响
EFFECTS OF FISH OIL REPLACEMENT BY VEGETABLE OILS IN FATTENING DIETS ON THE RELATED GENE EXPRESSION OF FATTY ACID METABOLISM OF ADULT MALE CHINESE MITTEN CRAB, ERIOCHEIRSINENSIS
摘要
Abstract
Fish oil is one of the major aquafeed ingredients, however, the world fish oil production could not meet the demand of global aquafeed industry. Thus, fish oil replacement have became a hot topic for the research and develop-ment of aquaculture. Chinese mitten crab Eriocheir sinensis is an important aquaculture species in China, and the pre-vious study has shown the dietary substitution of fish oil with vegetable oil negatively impact the gonadal development and nutritional quality of this species possibly via lipid metabolism. The present study investigated the expression pat-tern of fatty acid metabolism-related genes, including fatty acyl elongase (Es-FAE),Δ9 fatty acyl desaturase (Es-FAD9),Δ6 fatty acyl desaturase (Es-FAD6b), fatty acid-binding protein 3 (Es-FABP3), fatty acid-binding protein 9 (Es-FABP9) and fatty acid-binding protein 10 (Es-FABP10), and their expression by different fish oil replacement levels (i.e. 0, 25%, 50%, 75%and 100%) in the hepatopancreas of E. sinensis. The results indicated that Es-FAE, Es-FAD9 and Es-FAD6b had the highest expression levels in hepatopancreas but low expression levels in heart and haemo-lymph, and that Es-FABP3, Es-FABP9 and Es-FABP10 were mainly distributed in hepatopancreas, muscle, seminal and accessory gland whereas the relatively low expression levels in stomach, intestine and heart. The Es-FAE-mRNA ex-pression level in hepatopancreas significantly increased with the increasing dietary fish oil replacement levels. Diet 2# treatment had significantly higher Es-FAD6b-mRNA expression level than the Diet 3# treatment but no significant dif-ference among other treatments. Decreasing dietary fish oil contents reduced the expression of the Es-FAD9 in hepato-pancreas but without significant difference. (3) Diet 2# and Diet 3# but not other treatments significantly induced Es-FABP3-mRNA expression in hepatopancreas (P<0.05). Dietary fish oil replacement did not significantly regulate Es-FABP9 and Es-FABP10-mRNA expression but had trend of‘low-high-low’ in the hepatopancreas among the five treat-ments. These results indicated that hepatopancreas had the highest mRNA expression of the genes related to fatty acid metabolism and transportation, and that dietary fish oil replacement had significant effects on their expression.关键词
中华绒螯蟹/鱼油替代/育肥饲料/脂质代谢/脂肪酸结合蛋白Key words
Eriocheir sinensis/Fish oil replacement/Fattening diet/Fatty acid metabolism/Fatty acid-binding protein分类
水产学引用本文复制引用
刘泽华,吴旭干,龙晓文,赵磊,李嘉尧,成永旭..育肥饲料中植物油混合替代鱼油对中华绒螯蟹雄体脂肪酸代谢相关基因表达的影响[J].水生生物学报,2016,40(4):767-778,12.基金项目
上海市科学技术委员会科研项目(13320502100,13231203504) （13320502100,13231203504）
科技部港澳台科技合作专项项目(2014DFT30270) （2014DFT30270）
国家自然科学基金项目(31472287,31302159) （31472287,31302159）
上海高校水产学一流学科建设项目(2012-62-0908)资助[Supported by the Projects (13320502100 and 13231203504) from Shanghai Municipal Science and Technology Commission （2012-62-0908）
the Hongkong, Macao and Taiwan Science and Technology Cooperation Projects (2014DFT30270) （2014DFT30270）
a Grant from Nature Science Foundation of China (31472287 and 31302159) and the Shanghai Universities First-class Disciplines Project of Fisheries (No.2012-62-0908) from Shanghai Municipal Education Committee] （31472287 and 31302159）
