水生生物学报2016，Vol.40Issue(4)：779-792,14.DOI:10.7541/2016.102
饲料丙二醛对草鱼生长、肝胰脏及肠道结构和功能的影响
EFFECTS OF MDA ON THE GROWTH PERFORMANCE, STRUCTURE AND FUNCTION OF HEPATOPANCREAS AND INTESTINE OF GRASS CARP (CTENOPHARYNGODON IDELLUS)
摘要
Abstract
The study investigated effects of MDA on the growth performance, structure and function of hepatopancreas and intestine of grass carp (Ctenopharyngodon idellus) and evaluated effects between MDA and other products of oxi-dized fish oil. Five isonitrogenous and isoenergetic diets were prepared with soybean oil, fish oil and different level of MDA for a 72-day experiment. The results indicated that MDA and other products of oxidized fish oil both signifi-cantly increased the FCR of grass carp and decreased the SGR and PRR of grass carp (P<0.05). Only MDA signifi-cantly decreased the LRR (P<0.05). Moreover, MDA and other products of oxidized fish oil both significantly de-creased the TBA content in serum (P<0.05), and increased the content of TC, TG and MDA and the activity of SOD in serum (P<0.05). Only MDA significantly increased ALT content and decreased the radio between HDL and LDL in serum (P<0.05). MDA and other products of oxidized fish oil both significantly increased the lipid content of hepato-pancreas (P<0.05) and stimulated the oxidative stress of hepatopancreas. MDA damaged the morphology in hepatopan-creas cell and significantly decreased the number of nucleus of hepatopancreas cell (P<0.05), which lead to the obvi-ously trend of fibrosis of the hepatopancreas cell. MDA and other products of oxidized fish oil both significantly in-creased the number of goblet cells in the microvilli (P<0.05). To the permeability, serum D-lactic acid and endotoxin significantly increased because of the defect of the tight junction, the hyperplasia and edema of villi. In conclusion, MDA decrease the growth performance of grass carp by regulating the oxidative stress and lipid metabolism. MDA and other products of oxidized fish oil affect the hepatopancreas with different mechanism. Both MDA and other products of oxidized fish oil can mediate the function of intestine.关键词
血清/脂肪代谢/胆汁酸/线粒体/紧密连接Key words
Sreum/Fat Metabolism/Bile Acid/Morphology/Tight Junction分类
农业科技引用本文复制引用
陈科全,萧培珍,周亚琴,叶元土,蔡春芳,吴萍,黄雨薇,吴韬,林秀秀,罗其刚,张宝彤..饲料丙二醛对草鱼生长、肝胰脏及肠道结构和功能的影响[J].水生生物学报,2016,40(4):779-792,14.基金项目
国家自然科学基金项目(31172417) （31172417）
苏州市应用基础研究项目(SYN201316)资助[Supported by the National Natural Science Foundation of China (31172417) （SYN201316）
Applied Basic Research Programs of Suzhou City (SYN201316)] （SYN201316）
