水生生物学报2016，Vol.40Issue(4)：814-822,9.DOI:10.7541/2016.105
喂养不同饵料对匙吻鲟和鳙消化道微生物区系的影响
GUT MICROBIOTA OF BIGHEAD CARP (ARISTICHTHYSNOBILIS) AND PADDLEFISH (POLYODONSPATHULA) AFFECTED BY THEIR DIETS
摘要
Abstract
The gastrointestinal microbiota plays an important role in the growth, nutrition and well being of fishes and it would be necessary to know the microflora structure in the gastrointestinal tract and the influ-ence factors. In this background, we used nested polymerase chain reaction-denaturing gradient gel electro-phoresis (PCR-DGGE) and sequence analysis to investigate microbiota in the intestine of bighead carp and paddlefish. In this research, bighead carp was fed with live feed (BI-L) and compound feed (BI-C), and pad-dlefish was fed with live feed (PI-L, PS-L) and compound feed (PI-C, PS-C). A total of 16 bacterial species were identified by phylogenetic analysis. The Firmicutes group (31.25%, 5 OTU) totally dominated the bac-terial communities in bighead carp and paddlefish. The other identified bacterial species includedγ-Proteo-bacteria (25%, 4 OTU), Bacteroidetes (12.5%, 2 OTU), Fusobacteria (12.5%, 2 OTU), Cyanobacteria (6.25%, one OTU), Actinobacteria (6.25%, one OTU) andβ-Proteobacteria (6.25%, one OTU). The higher similarities (above 49%in paddlefish and 59%in bighead carp) of the DGGE band patterns in intestinal bacteria between two groups feeding with different feed indicated that there are more stable microbial com-muni ties existed in these specifically ecological niches in both bighead carp and paddlefish. However, the microflora structure in the stomach has considerable difference in the two groups of paddlefish feeding with different feed, probably indicating that the community structures in stomach of paddlefish were affected by diet easily and significantly.关键词
鳙/匙吻鲟/微生物/DGGE/消化道Key words
Bighead carp/Paddlefish/Microbiota/DGGE/Gastrointestinal tract分类
农业科技引用本文复制引用
王琴,熊邦喜,罗国强,苏艳秋,刘海燕..喂养不同饵料对匙吻鲟和鳙消化道微生物区系的影响[J].水生生物学报,2016,40(4):814-822,9.基金项目
Supported by State Key Development Program (973) for Basic Research of China (Grant No.2009CB118706) （973）
