水生生物学报2016，Vol.40Issue(4)：823-829,7.DOI:10.7541/2016.106
饲料中添加抗菌肽Surfactin对吉富罗非鱼肠道健康的影响
EFFECT OF DIETARY ANTIMICROBIAL PEPTIDES-SURFACTIN SUPPLEMENTATION ON PARAMETERS OF INTESTINAL HEALTH INDICES OF GENETICALLY IMPROVED FARMED TILAPIA (GIFT, OREOCHROMIS NILOTICUS)
摘要
Abstract
A 7-week feeding trial was conducted to evaluate effects of antimicrobial peptides-surfactin on intestinal health indices of genetically improved farmed tilapia (GIFT, Oreochromis niloticus). Three hundred and twenty fish with initial average weight of (12.01±0.12) g were randomly divided into four treatment groups with four replicates and 20 fish in each replicate. The fish were fed experimental diets supplemented with 0 (control group), 50, 100, and 200 mg/kg antimicrobial peptides-surfactin, respectively. Surfactin supplementation increased intestinal folds height of GIFT (P<0.05), but did not impact the muscular thickness (P>0.05). Surfactin supplementation significantly decreased the number of Escherichia coli of GIFT (P<0.05) and significantly increased the number of Lactobacillus (P<0.05). There was no significant difference in the total bacterial number among all treatment groups (P>0.05). Surfactin supple-mentation significantly increased the total antioxidant capacity level and activities of glutathion peroxidase and super-oxide dismutase (except 200 mg/kg Surfactin supplementation group) (P<0.05) but significantly decreased the content of malondialdehyde (P<0.05). Surfactin supplementation had no effect on catalase activity (P>0.05). The intestinal health parameters of 50 mg/kg antimicrobial peptides-surfactin supplementation group were the best among all supple-mentation groups. In conclusion, surfactin supplementation at 50 mg/kg could improve intestinal health status of GIFT by increasing the intestinal folds height and regulating intestinal microbial population and antioxidant ability.关键词
抗菌肽/吉富罗非鱼/肠道健康Key words
Antimicrobial peptides/Genetically improved farmed tilapia/Intestinal health分类
农业科技引用本文复制引用
翟少伟,史庆超,陈学豪..饲料中添加抗菌肽Surfactin对吉富罗非鱼肠道健康的影响[J].水生生物学报,2016,40(4):823-829,7.基金项目
“十二五”农村领域国家科技计划课题“功能性肽产品的创制与应用”(2013BAD10B03)资助[Supported by Key Project of National Science and Technology R&D Program During the Twelfth Five-Year Plan Period“Development and Application of Functional Peptide Products”(2013BAD10B03)] （2013BAD10B03）
