水生生物学报2016，Vol.40Issue(4)：843-852,10.DOI:10.7541/2016.109
基于稳定同位素分析不同蛋白源对大菱鲆生长的影响
THE NUTRIONAL CONTRIBUTIONS OF DIETARY PROTEIN SOURCES TO GROWTH OF TURBOT (SCOPHTHATMUSMAXIMUSL.):EVIDENCE FROM STABLE ISOTOPE ANALYSIS (SIA)
摘要
Abstract
To investigate effects of dietary protein sources to turbot growth (Scophthatmus maximus L.), we substituted fish meal by plant protein blend (corn gluten and soy protein concentrate 1∶1 isonitrogenous mixed) in diets and deter-mined nitrogen stable isotope ratios of diet and tissue. Four isonitrogenous and isoenergetic diets with different replace-ment 0, 10%, 30%and 50%fish meal protein by plant protein blend were formulated and correspondingly named as CS0, CS10, CS30 and CS50, respectively, for a 56 days experiment with 3 replicates in each group. Our results showed that food intake (FI) and specific growth rate (SGR) in CS50 were significantly lower but the feed coefficiency was higher compared with other groups. The turnover rates of nitrogen stable isotope were significantly different between each diet (P<0.05) with the highest turnover rate in the control group. Liver responded more quickly to the diet com-pared with muscle. Nitrogen half-lives (T0.5) of muscle were significantly higher than those of liver (P<0.05). The pro-portional contributions of growth to nitrogen turnover were 57.33%—73.33%for muscle and 29.17%—36.10%for li-ver, respectively. Based on turnover model, the fractionation (Δ) were ranged from 1.83‰to 2.83‰. Calculated by Iso-source software, the contributions of fish meal, corn gluten and SPC to tissue growth and metabolism of turbot were 80%, 7%and 13%for CS10, 62%, 11%and 27%for CS30, 46%, 13%and 41%for CS50, respectively. The result showed that stable isotopic turnover was driven by both tissue replacement in the form of metabolism and tissue in-crease in the form of growth, the proportional contritetiens of which between tissues exhibited significant discrepancy;nitrogen stable isotope, turnover rate of liver was faster than that of muscle, without, the half-time of liver was also faster;the contribution rate of dietary corn protein to the protein growth of the turbot was lower than the SPC.关键词
大菱鲆/氮稳定同位素/生长/代谢/周转模型Key words
Scophthatmus maximus L./Nitrogen stable isotope/Growth/Metabolism/Turnover model分类
农业科技引用本文复制引用
李忠清,夏斌,张利民,王际英,李宝山,孙永智,王明辉..基于稳定同位素分析不同蛋白源对大菱鲆生长的影响[J].水生生物学报,2016,40(4):843-852,10.基金项目
水生动物营养与饲料创新示范平台项目(201501006) （201501006）
国家海洋公益性行业科研专项(201205025) （201205025）
山东省科技发展计划(2014GHY115006)资助[Supported by the Aquatic Animal Nutrition and Feed Research and Innovation Demonstration Platform (201501006) （2014GHY115006）
Public Science and Technology Research Funds of Ocean from the State Oceanic Administration, People’s Republic of China (201205025) （201205025）
Shandong Science and Technology Plan Project (2014GHY115006)] （2014GHY115006）
