水生态学杂志2016，Vol.37Issue(3)：49-55,7.DOI:10.15928/j.1674-3075.2016.03.007
三峡水库淹没水深对消落带植物牛鞭草和狗牙根生长及抗氧化酶活性的影响
Growth and Antioxidant Enzyme Activity in Hemarthria altissima and Cynodon dactylon in Response to Water Depth in the Water Level Fluctuation Zone of Three Gorges Reservoir
摘要
Abstract
The water level fluctuation zone (WLFZ)is the most common and important feature of regulated rivers. The fluctuation zone in Three Gorges Reservoir (TGR)since impoundment reached 175 m on October 26,2010 is large,resulting in a WLFZ of up to 30m.The TGR operating cycle has also reversed the natural hydrologic rhythm, exposing land in summer (flood season)and submerging it in winter (dry season).Research has shown that the o-riginal vegetation in the WLFZ,especially trees and shrubs,did not survive because they could not tolerate winter submergence and summer drought and herbs have become the most abundant type of vegetation,with absolute domi-nance by perennial herbs such as Hemarthria altissima and Cynodon dactylon.It is important to examine the eco-physiological adaption of dominant species in the WLFZ during the submergence period to provide information for effectively restoring vegetation in the WLFZ.To investigate growth and antioxidant enzyme activity of dominant plants in the WLFZ of TGR,an in situ experiment using H.altissima and C.dactylon cultivation was carried out at four water depths (0,2,5 and 15 m)in Kai County,Chongqing City from November,2014 to May,2015.All plants were cultivated in plastic pots (20 cm ×30 cm ×30 cm)hung from a floating platform and the height,cov-er,germination number,biomass,total protein,malondiadehyde (MDA),and the enzyme activities of superoxide dismutase (SOD)and peroxidase (POD)in the root system of H.altissima and C.dactylon were measured on days 30,60 and 180.Water quality parameters were simultaneously measured,including turbidity,illumination, temperature,conductivity,dissolved oxygen and pH.Total protein was correlated with MDA and antioxidant en-zyme activities.Results show that height,cover,sprout number,biomass,total protein,enzyme activity of SOD and the MDA content of submerged H.altissima and C.dactylon were all less than for unsubmerged plants,but POD activity in the root system increased.All differences,except for biomass and MDA content,were gradually significant with the submergence water depth.The positive correlation between MDA content and SOD activity was also significant.The correlation coefficients between SOD and POD and between MDA and SOD were lowest at the water depth of 15 m,so submergence may play a role in the relationship between total protein content and antioxi-dant enzyme activity.Correlation coefficients among enzymes of C.dactylon were higher than those of H.altissima. These results provide insight on the physiological adaptation of herbal plants to submergence and support efforts to restore vegetation in the WLFZ of reservoirs.关键词
狗牙根/牛鞭草/生长/抗氧化酶活性/消落带/三峡水库/原位试验Key words
Hemarthria altissima/Cynodon dactylon/growth/antioxidant enzyme activities/water-level-fluctua-ting zone/Three Gorges Reservoir/in situ experiment分类
资源环境引用本文复制引用
张志永,程丽,李春辉,胡红青,万成炎..三峡水库淹没水深对消落带植物牛鞭草和狗牙根生长及抗氧化酶活性的影响[J].水生态学杂志,2016,37(3):49-55,7.基金项目
国家科技支撑计划课题（2012BAC06B04）；国家水体污染控制与治理重大专项（2013ZX07104-004）；三峡后续工作科研项目（2013HXKY2-3）。 （）
