生物加工过程2016，Vol.14Issue(4)：1-4,11,5.DOI:10.3969/j.issn.1672-3678.2016.04.001
米根霉两段式利用板栗栗苞生产富马酸
Fumaric acid production from autohydrolysis-pretreated Chinese chestnut shell by Rhizopus oryzae in a two-stage fermentation
摘要
Abstract
Fumaric acid production from autohydrolysis⁃pretreated Chinese chestnut shell by Rhizopus oryzae was investigated in a two⁃stage fermentation. Autohydrolysis pretreatment of Chinese chestnut shell was carried out at 220 ℃. Enzymatic hydrolysis yield of pretreated Chinese chestnut shell was over 95%based on 50 g/L substrate loading using a cellulase loading of 50 FPIU/g cellulose. Rhizopus oryzae could grow and obtain 4. 5 g/L fungal biomass in proliferation medium containing xylose.Pre⁃cultured Rhizopus oryzae was used for production of fumaric acid from enzymatic hydrolysate of pretreated Chinese chestnut shell. Fumaric acid of 15. 78 g/L was obtained after 96 h fermentation with a yield of 0. 34 g/g.关键词
板栗栗苞/自水解预处理/米根霉/两段式发酵/富马酸Key words
Chinese chestnut shell/autohydrolysis/Rhizopus oryzae/two-stage fermentation/fumaric acid分类
化学工程引用本文复制引用
李鑫,周瑾,虞慧,欧阳水平..米根霉两段式利用板栗栗苞生产富马酸[J].生物加工过程,2016,14(4):1-4,11,5.基金项目
江苏省自然科学基金（BK20131426）；江苏省高校自然科学研究重大项目（14KJA220003）；江苏省重点研发计划项目 （）
