生物加工过程2016，Vol.14Issue(4)：5-11,7.DOI:10.3969/j.issn.1672-3678.2016.04.002
海藻酸钙凝胶颗粒固定化抗冻酵母AFY 1的机制分析及条件优化
Mechanism analysis and condition optimization for the immobilization of freeze-tolerant yeast AFY-1 within Ca-alginate gel particles
摘要
Abstract
The present study aimed at immobilizing freeze⁃tolerant yeast AFY⁃1 within Ca⁃alginate gel particles,and clarifying its mechanisms. The yeast was immobilized under various conditions with different Na⁃alginate concentrations, CaCl2 concentrations and solidification times, and then the ethanol fermentations by the immobilized yeasts were carried out under a pre⁃determined condition. The interrelation among the fermentation ability, the yeast growth and the permeability of gel particles was analyzed. The immobilization conditions were optimized through the surface response experiments. The experimental results showed that the ethanol yield increased proportionally with increasing permeability of gel particles, whereas the yeast growth did not change significantly with the immobilization conditions. When the immobilization condition was at a Na⁃alginate mass concentration of 1. 45%,a CaCl2 mass concentration of 17. 45% and a solidification time of 1. 09 h,the immobilized yeast had an ethanol yield of 24. 1%,which was higher than 20. 9% obtained by free yeast. In addition,the immobilized yeast grew faster than the free yeast did. The total cell number in each Erlenmeyer flask (250 mL) increased from 5. 0×108 to 11. 2×108 during the ethanol fermentation by the immobilized yeast,while it increased from 5. 0×108 to 7. 5×108 only during the ethanol fermentation by the free yeast.关键词
酵母/固定化/海藻酸钙凝胶/颗粒/通透性/发酵Key words
yeast/immobilization/Ca-alginate gel/particle/permeability/fermentation分类
生物工程引用本文复制引用
尤业兵,缪冶炼,陈介余,季萌,孙研,许琳..海藻酸钙凝胶颗粒固定化抗冻酵母AFY 1的机制分析及条件优化[J].生物加工过程,2016,14(4):5-11,7.基金项目
国家重点基础研究发展计划（973计划）（2011CBA00807）；江苏省大学生创新项目 （）
