生物加工过程2016，Vol.14Issue(4)：12-16,5.DOI:10.3969/j.issn.1672-3678.2016.04.003
响应面法优化红球菌产生物表面活性剂的发酵条件
Optimization of biosurfactant production by Rhodococcus SY095 with response surface methodology
摘要
Abstract
To improve the biosurfactant production by Rhodococcus SY095, single factor experiment and response surface methodology were applied to optimize the fermentation conditions. Temperature, rotate speed,initial pH and inoculum amount were studied. Box⁃Behnken experiment was designed by Expert 8. 06.The quadratic regression model with variables such as temperature,rotate speed and initial pH was established. Finally, the optimal fermentations conditions were determined as follows: fermentation temperature 29. 5 ℃,rotate speed 158 r/min,initial pH 7. 42. Under the optimal conditions,the surface tension of fermentation supernatant reached 29. 854 mN/m,and the biosurfactant yield was 3. 31 g/L.In a 70⁃L fermentor,the biosurfactant yield reached 9. 28 g/L,2. 8 times higher than that cultured in shaking flask.关键词
生物表面活性剂/响应面法/发酵条件优化/Box-Behenken设计Key words
biosurfactant/response surface methodology/fermentation optimization/Box-Behenk design分类
生物工程引用本文复制引用
张晓青,郝建安,司晓光,姜天翔,王静,张雨山..响应面法优化红球菌产生物表面活性剂的发酵条件[J].生物加工过程,2016,14(4):12-16,5.基金项目
海洋公益性行业科研专项项目（20130522）；中央级公益性科研院所基本科研业务费专项资金资助项目 （）
