生物加工过程2016，Vol.14Issue(4)：29-32,75,5.DOI:10.3969/j.issn.1672-3678.2016.04.006
碳、氮源对纳豆芽孢杆菌R127发酵产维生素K2的影响
Effects of carbon and nitrogen source on vitamin K2 production by Bacillus subtilis natto R127
摘要
Abstract
Vitamin K2 is an essential lipid⁃soluble coagulation vitamin that plays an important role in blood clotting and anti⁃osteoporosis. We studied the effects of different content of carbon and nitrogen source on the growth of Bacillus subtilis natto,a Vitamin K2 producer screened by our laboratory.Factors studied include substrate consumption and secretion of vitamin K2.Results show that the optimal glycerol concentration was 50 g/L, and the yield of vitamin K2 reached 15. 8 mg/L. An inverse correlation was observed between biomass and the concentration of nitrogen source,whereas the yield of vitamin K2 was proportional to the nitrogen concentration. In addition, vitamin K2 was a water⁃soluble compound metabolite in the fermentation process,the secretory rate was about 68. 8%.关键词
维生素K2/纳豆芽孢杆菌/氮源/碳源Key words
vitamin K2/Bacillus subtilis natto/nitrogen source/carbon source分类
生物工程引用本文复制引用
周子民,罗苗苗,邱宏伟,王丽,张绮悦,任路静,黄和..碳、氮源对纳豆芽孢杆菌R127发酵产维生素K2的影响[J].生物加工过程,2016,14(4):29-32,75,5.基金项目
国家高技术研究发展计划（863计划）（2014AA021701）；国家自然科学基金（21306085）；江苏省自然科学基金（BK2012424）；高等学校博士学科点专项科研基金 （）
