生物加工过程2016，Vol.14Issue(4)：33-36,4.DOI:10.3969/j.issn.1672-3678.2016.04.007
新型海洋源β半乳糖苷酶在毕赤酵母中的表达与酶学性质
Expression and characterization of a novelmarine β-galactosidase in Pichia pastoris
摘要
Abstract
Using Pichia pastoris expression system, we constructed a recombinant P. pastoris strain producing marine β⁃galactosidase that was subsequently characterized. The recombinant enzyme had optimum pH between 7. 0 and 8. 0. Its optimum temperature was 45 ℃, and over 50% relative activity below 50 ℃.It was stabile in the presence of Fe2+,Na2+,K2+ and Ca2+. Broad stable pH and temperature range and tolerance to metal ions make this recombinant enzyme suitable in various applications.关键词
半乳糖苷酶/毕赤酵母/重组Key words
galactosidase/Pichia pastoris yeast/recombinant分类
生物科学引用本文复制引用
阎金勇,董敬霞,査根晗,高云,刘小宇,缪明永..新型海洋源β半乳糖苷酶在毕赤酵母中的表达与酶学性质[J].生物加工过程,2016,14(4):33-36,4.基金项目
国家高技术研究发展计划（863计划） （）
