生物加工过程2016，Vol.14Issue(4)：37-42,6.DOI:10.3969/j.issn.1672-3678.2016.04.008
载体两性电解质修饰对固定化γ谷氨酰转肽酶pH耐受性和催化活性的影响
Improving pH tolerance and catalytic properties of immobilizedγ-glutamyltranspeptidase by coated with carrier ampholyte
摘要
Abstract
In order to improve pH tolerance of γ⁃glutamyltranspeptidase ( GGT ) , recombinant γ⁃glutamyltranspeptidase ( GGT ) from Bacillus subtilis was immobilized onto silylated mesoporous TiO2 whiskers(sMTw). Then it was modified with carrier ampholyte(Pharmalyte pH 8. 0 to 10. 5,CA). The pH tolerance of modified immobilized enzyme(sMTw⁃GGT⁃CA)was greatly improved in comparing with the free GGT, stable under pH ranging from 6. 0 to 11. 0. Furthermore, the thermal stability of sMTw⁃GGT⁃CA was also higher than that of the free GGT, with optimal temperature as high as 50 ℃. The thermal inactivation energy( Ed ) of MTwA⁃GGT⁃CA was calculated to be 49. 88 kJ/mol. Also,the Km of MTwA⁃GGT⁃CA towards GpNA was 0. 579 mmol/L, very close to that of the free GGT.关键词
γ 谷氨酰转肽酶/载体两性电解质/修饰/pH耐受性/催化活性Key words
γ-glutamyltranspeptidase/carrier ampholyte/modification/pH tolerance/catalytic properties分类
生物工程引用本文复制引用
韦敏,姚忠,叶丽静,王浩绮,倪芳,周治,仲兆祥,孙芸..载体两性电解质修饰对固定化γ谷氨酰转肽酶pH耐受性和催化活性的影响[J].生物加工过程,2016,14(4):37-42,6.基金项目
国家自然科学基金（21206072）；江苏省自然科学基金 （）
