生物技术通报2016，Vol.32Issue(7)：81-86,6.DOI:10.13560/j.cnki.biotech.bull.1985.2016.07.012
香蕉水通道蛋白 SIP2-1基因的克隆和表达分析
Molecular Cloning and Expression Analysis of Aquaporins SIP2-1 Gene from Banana
摘要
Abstract
In the present study,we isolated an aquaporin gene from banana,and designated as MaSIP2-1. MaSIP2-1’s complete ORF was 717 bp,and it encoded 239 amino acids. Alignment of amino acid sequences and phylogenetic analysis indicated that the protein encoded by MaSIP2-1 was in high similarity with AQP-encoding protein in the other known plants ;and highly homologous with amino acid sequences in Musa acuminate,Elaeis guineensis,Jatropha curcas,and Camellia sinensis by 98%,74%,65%,and 63%,respectively. Organ-specific analysis showed that MaSIP2-1 constitutively expressed in roots,stems,leaves,flowers and fruits,and the highest in stems. Stress analysis demonstrated that MaSIP2-1 responded to the stress such as the drought,salt and waterlogging.关键词
水通道蛋白/SIP/香蕉/生物信息学/胁迫/荧光定量 PCRKey words
aquaporins/SIP/banana/bioinformatics/stress/real-time PCR引用本文复制引用
许奕,侯晓婉,徐碧玉,金志强,胡伟,宋顺..香蕉水通道蛋白 SIP2-1基因的克隆和表达分析[J].生物技术通报,2016,32(7):81-86,6.基金项目
国家自然科学基金项目（31071788，31501371），海南省自然科学基金项目（314099），现代农业产业技术体系建设专项资金项目（CARS-32），“十二五”农村领域国家科技计划项目 （）
