生物技术通报2016，Vol.32Issue(7)：87-92,6.DOI:10.13560/j.cnki.biotech.bull.1985.2016.07.013
内生菌次生代谢产物提高玉米渗透胁迫抗性的表达谱分析
Expression Profile Analysis of Maize Resistance Under Osmotic Stress Induced by Secondary Metabolites of Endophytes
摘要
Abstract
Genechip technology was used to analyze the expression profile of differential genes in corn treated by metabolites of endophyte under 10% PEG osmotic stress. The results showed that there were 441 differentially expressed genes identified in this study including 147 up-regulated genes and 294 down-regulated genes,participating in 21 metabolic pathways. The genes with differential expressions were classified by GO-ranking methods through CapitalBio Molecule Annotation System V 3.0(MAS). Among them 45% participated in various biological processes,18% were related to synthesis of cellular components,the coded products of 36% genes were related to performing a variety of molecular function,and 1% corresponded with other unknown sequences in GenBank. Thus the role of these genes still needs further study. Pathways analysis using the KEGG pathway database showed the differentially expressed genes broadly were involved in basic metabolism, energy metabolism,secondary metabolism and signal transduction pathways. The expression profile analysis indicate that the effects of secondary metabolites on corn under osmotic stress are synergistical processes by many genes in many pathways.关键词
内生真菌/玉米/渗透胁迫/基因表达谱Key words
endophyte/maize/osmotic stress/gene expression profile引用本文复制引用
王娜,龚娜,刘国丽,马晓颖,杨镇,杨涛..内生菌次生代谢产物提高玉米渗透胁迫抗性的表达谱分析[J].生物技术通报,2016,32(7):87-92,6.基金项目
辽宁省农业科技攻关项目（2011215005），辽宁省科学事业公益研究基金项目 （）
