生物技术通报2016，Vol.32Issue(7)：106-111,6.DOI:10.13560/j.cnki.biotech.bull.1985.2016.07.016
拮抗多种植物病原真菌 Streptomyces triostinicus C2的分离与鉴定
Isolation and Identification of Streptomyces triostinicus C2 Antagonizing on a Variety of Plant Pathogenic Fungi
摘要
Abstract
The research purposes of this work are to isolate and screen a strain against phytopathogenic fungi. The antagonistic strain was isolated and screened using plate confrontation culture with Rhizoctonia solani as indicator,and was further identified on the basis of morphological characteristics,physiological and biochemical characterization,and 16S rDNA sequence analysis. From 18 soil samples collected from Yunnan,Guangdong,Anhui,Hubei,and Jiangxi Provinces of China,the isolated actinomycete strain C2 presented a strong inhibitory effect on R. solani,and it was preliminarily identified as Streptomyces triostinicus,designated as Streptomyces strain C2 here. The bioassay results showed that Streptomyces strain C2 possessed a broad-spectrum inhibitory effect on a range of plant fungi such as R. solani,Pyricularia grisea,Colletotrichum gloeosporioides,Fusarium oxysporum f. sp. niveum,and Penicillium citrinum,and the inhibition percentage was 49.78%,56.62%,80.96%,18.59%,and 94.10%,respectively. Conclusively,Streptomyces strain C2 was an antagonistic strain with broad-spectrum in suppressing the growth of various plant fungal pathogens.关键词
分离与鉴定/放线菌菌株 C2/植物病原真菌/广谱拮抗Key words
isolation and identification/actinomycete strain C2/phytopathogenic fungi/broad-spectrum antagonist引用本文复制引用
彭卫福,吴志明,陈未,曾勇军,李昆太..拮抗多种植物病原真菌 Streptomyces triostinicus C2的分离与鉴定[J].生物技术通报,2016,32(7):106-111,6.基金项目
国家科技支撑计划项目（2011BAD16B0402，2013BAD07B12），江西省青年科学家培养对象计划项目（20142BCB23025），国家级大学生创新创业训练计划项目 （）
