生物技术通报2016，Vol.32Issue(7)：146-151,6.DOI:10.13560/j.cnki.biotech.bull.1985.2016.07.022
红花脂质转运蛋白基因的克隆及表达分析
Cloning and Expression Analysis of Lipid Transfer Protein LTP Gene in Carthamus tinctorius
摘要
Abstract
LTP(Lipid transfer protein)gene was cloned,its bioinformatics and expression were analyzed,providing the basis for the study the role of LTP gene in safflower resisting stresses. The sequence of LTP gene was cloned by RT-PCR technique,the protein characteristics were analyzed using bioinformatics,and the phylogenetic tree of LTP for safflower and other species was constructed. The expressions of LTP gene in the different tissues were analyzed using Real time-PCR. Sequence analysis showed that ORF of LTP was 294 bp, encoding a protein of 97 amino acids with a molecular weight of 7.46 kD,isoelectric point of 8.91. Safflower LTP protein contained a long signal peptide sequence of 29 amino acid residues,and the protein contained a serine phosphorylation site. Tertiary structure prediction indicated that the protein was a simple structure of 3 α-helix and 1 β-turn twining in random coils. Molecular evolution showed that the evolutionary relationship of LTP between Brassica of cruciferous and safflower was the most similar. Tissue-specific expression analysis of safflower LTP by fluorescence quantitative PCR showed that the gene expression levels in different tissues presented a significant difference,while high expression in seeds and flowers,and low expression in other tissues.关键词
脂质转运蛋白/红花/荧光定量 PCR/生物信息学分析Key words
lipid transfer protein/safflower/Real-time PCR/bioinformatics analysis引用本文复制引用
韩怡来,李校堃,崔琪,武云云,顾天瑶,滕孝花,胡人阁,吴贯达,官丽莉,李海燕..红花脂质转运蛋白基因的克隆及表达分析[J].生物技术通报,2016,32(7):146-151,6.基金项目
国家高技术研究发展计划（“863”）项目（2011AA100606），吉林省教育厅“十三五”科学技术研究项目重点项目（吉教科合字［2016］179号），国家自然科学基金资助项目（31201237），吉林农业大学大学生创新创业项目（201610193046，201410193045），吉林省科技厅中青年领军人才及优秀创新团队项目（20111815），教育部博士点基金-青年教师基金项目 （）
