山西农业大学学报（自然科学版）2016，Vol.36Issue(7)：467-471,505,6.
鸡大肠杆菌FimH基因C端结构域克隆及特性分析
Cloning and characterization of C terminal domain of avian E .coli ’Fim H gene
摘要
Abstract
Objective]Through further study of Fim H ,the the main structure gene of avian Escherichia coli type I , the research aimed to provide necessary theoretical foundation for pathogenic mechanism of avian E .coli and develop-ment of gene engineering vaccinE.[Methods]Based on the published C terminal domain nucleotide sequence of Fim H gene for reference,this research designed and synthesized primers,amplified three strains of the relevant Fim H genes in avian E .coli including JS1,JS2 and JS6,used Lasergene software for sequence alignment and protein structure pre-diction,and the evolutionary tree was drew,etc.[Results]The results showed that the homology of gene nucleotide sequences between Reference strain and JS1,JS2 or JS6 were 97.3%,97.7% and 97.3%,and their amino acid se-quences was 95.3%,97.6% and 95.3%,respectively.Although their Fim H antigenic nucleotide sequences between Reference strain and JS1,JS2 or JS6 were 97.3%,97.7% and 97.3%,and their amino acid sequences was 95.3%, 97.6% and 95.3%,respectively.Although their Fim H antigenic determinants are basically similar,amino acids changes at 78 th and 79 th caused the different antigenic determinant at C terminal domain of Fim H ,which indicated that amino acid variation of Fim H sequences might have the minor effect on the antigenic property of Fim H .Further-more,the evolutionary tree analysis showed the close evolutionary relationship among three isolated strains and the do-mestic avian E .coli strains.[Conclusion]The results indicated that the Fim H gene of avian E .coli had no significant variation,which makes important foundation for further research on molecular mechanism of avian E .coli and the con-trol strategies against pathogenic avian E .coli.关键词
鸡大肠杆菌/Fim H 基因/克隆/特性分析Key words
Avian E .coli/Fim H gene/Gene clone/Characterization analysis分类
农业科技引用本文复制引用
白宇琛,谢金峰,周广防,冯秀丽..鸡大肠杆菌FimH基因C端结构域克隆及特性分析[J].山西农业大学学报（自然科学版）,2016,36(7):467-471,505,6.基金项目
江苏省自然科学基金青年基金项目(BK20130682) （BK20130682）
江苏省高校优势学科建设工程资助项目(PAPD) （PAPD）
