山西农业大学学报（自然科学版）2016，Vol.36Issue(7)：472-476,5.
2株山西 IBV 分离株的生物学特性研究
Biological characteristics of two strains of Shanxi IBV isolates
摘要
Abstract
Objective]The aim was to control the epidemic of infectious bronchitis.[Methods]The biological characteris-tics of IBV-21 and IBV-22 were tested by infection of half quantity (EID50),chicken embryos small test and interfer-ence NDV LaSota copy test and chicken embryo TOCs experiment.We verified by RT-PCR amplification.[Results] The results showed that the EID50 of IBV-21 was 5×10 -4.6 1 6·mL-1 ,IBV-22 was 5×10 -6.5·mL-1 .Two strains had poison to chicken embryos that to be a little change as a result,the NDV LaSota interfered markedly,and chicken em-bryo TOCs was showed the lesions.The RT-PCR amplified S1 gene of 2 isolates,and stripe size was about 293 bp. [Conclusion]The biological characteristics showed that the separation of two viruses which was not existing vaccines had obvious pathogenic role in chicken embryos,and laid the foundation in local IBV vaccine development.关键词
IBV/生物学特性/RT-PCR/分离鉴定Key words
IBV/Biological Characteristics/RT-PCR/Separation Identification分类
农业科技引用本文复制引用
高诗敏,邓瑞林,闫芳,马海利,化丽珍,田文霞,高文伟..2株山西 IBV 分离株的生物学特性研究[J].山西农业大学学报（自然科学版）,2016,36(7):472-476,5.基金项目
山西农业大学科技创新基金(412579) （412579）
